PXD047775
PXD047775 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dataset Accompanying "Proteomic comparison of the organic matrices from parietal and base plate of the acorn barnacle Amphibalanus amphitrite" |
Description | ABSTRACT: Acorn barnacles are efficient colonizers on a wide variety of marine surfaces. As they proliferate on critical infrastructure, their settlement and growth have deleterious effects on performance. To address acorn barnacle biofouling, research has focused on the settlement and adhesion processes with the goal of informing the development of novel coatings. This effort has resulted in the discovery and characterization of several proteins found at the adhesive substrate interface, i.e. cement proteins, and a deepened understanding of the function and composition of the biomaterials within this region. While the adhesive properties at the interface are affected by the interaction between the proteins, substrate, and mechanics of the calcified base plate, little attention has been given to the interaction between the proteins and the cuticular material present at the substrate interface. Here, the proteome of the organic matrix isolated from the base plate of the acorn barnacle Amphibalanus amphitrite is compared with the chitinous and proteinaceous matrix embedded within A. amphitrite parietal plates. The objective was to gain an understanding of how the basal organic matrix may be specialized for adhesion via an in-depth comparative proteome analysis. In general, the majority of proteins identified in the parietal matrix were also found in the basal organic matrix, including nearly all those grouped in classes of cement proteins, enzymes and pheromones. However, the parietal organic matrix was enriched with cuticle-associated proteins, of which ~30% of those identified were unique to the parietal region. In contrast, ~30-40% of the protease inhibitors, enzymes, and pheromones identified in the basal organic matrix were unique to this region. Not unexpectedly, nearly 50% of the cement proteins identified in the basal region were significantly distinct from those found in the parietal region. The wider variety of identified proteins in the basal organic matrix indicates a greater diversity of biological function in the vicinity of the substrate interface where several processes related to adhesion, cuticle formation, and expansion of the base synchronize to play a key role in organism survival. |
HostingRepository | MassIVE |
AnnounceDate | 2023-12-12 |
AnnouncementXML | Submission_2023-12-12_20:01:57.010.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | J Hervey |
SpeciesList | scientific name: Amphibalanus amphitrite; |
ModificationList | unknown modification; unknown modification |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-12-12 19:41:55 | ID requested | |
⏵ 1 | 2023-12-12 20:01:57 | announced |
Publication List
Schultzhaus JN, Hervey WJ IV, Fears KP, Spillmann CM. Proteomic comparison of the organic matrices from parietal and base plate of the acorn barnacle Amphibalanus amphitrite. Open Biology. (submitted, under final review). |
Keyword List
submitter keyword: Amphibalanus amphitrite, cement protein, mass spectrometry, cuticle, chitin binding |
Contact List
J Hervey | |
---|---|
contact affiliation | NRL |
contact email | judson.hervey@nrl.navy.mil |
lab head | |
J Hervey | |
contact affiliation | NRL |
contact email | judson.hervey@gmail.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093654/ |