PXD047758

PXD047758 is an original dataset announced via ProteomeXchange.

Title	Analysis of fluorophore-labeled human cytochrome b5
Description	Cytochrome b5 (b5) is a small accessory protein involved in several reactions. The interaction of b5 with its partner proteins, e.g., cytochrome P450 enzymes, has received much attention and has been the subject of numerous studies. In order to obtain further insights into protein-protein interactions of b5, we modified it with a green fluorophore, Alexa 488, which has a maleimide linker. The labeling was applied to both WT (without Cys) and the T70C mutant. This study revealed labeled sites in WT b5 and confirmed the Cys-selective labeling in the T70C mutant.
HostingRepository	PRIDE
AnnounceDate	2024-06-22
AnnouncementXML	Submission_2024-06-22_08:23:35.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristie Lindsey Rose
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-12 08:00:23	ID requested
⏵ 1	2024-06-22 08:23:36	announced

10.1016/j.jbc.2024.105688;

Tateishi Y, Webb SN, Li B, Liu L, Lindsey Rose K, Leser M, Patel P, Guengerich FP, residues involved in binding and stimulation of cytochrome P450 17A1 17,20-lyase. J Biol Chem, 300(3):105688(2024) [pubmed]

submitter keyword: Cytochrome b5, Fluorophore, Human, Alexa 488

Kristie Lindsey Rose
contact affiliation	Vanderbilt University Proteomics Core
contact email	kristie.rose@vanderbilt.edu
lab head
Kristie Lindsey Rose
contact affiliation	Vanderbilt university
contact email	kristie.rose@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD047758

PRIDE project URI

• PRIDE
  1. PXD047758
     1. Label: PRIDE project
     2. Name: Analysis of fluorophore-labeled human cytochrome b5