The nutritional value of goat milk varies depending on the lactation time(Y. Zhang, Zheng, Liu, & Liu, 2020). Tandem mass tagging (TMT) was employed in this study to explore the differential proteome of mature milk and terminal milk from Guanzhong dairy goats. Goat mature milk was beneficial in the treatment and improvement of malaria patients, as well as in the treatment of pancreatic cancer patients. Terminal milk has been proven to promote angiogenesis, boost antibacterial and anti-inflammatory factor activity, and inhibit tumor formation. These bioinformatics investigations will provide new insights into the physiological activities of proteins in goat milk from Guanzhong dairy goats, as well as a scientific foundation for future study into the creation of specialized functionalised dairy products, such as cancer prevention.