PXD047722

PXD047722 is an original dataset announced via ProteomeXchange.

Title	Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma
Description	The WRAMP structure is a protein network associated with tail-end actomyosin contractility, membrane retraction, and directional persistence during cell migration. A marker of WRAMP structures is melanoma cell adhesion molecule (MCAM) which dynamically polarizes to the cell rear. However, factors that mediate MCAM polarization are still unknown. In this study, BioID using MCAM as bait identifies the ERM family proteins, moesin, ezrin, and radixin, as WRAMP structure components. We also present a novel image analysis pipeline, Protein Polarity by Percentile (“3P”), which classifies protein polarization using machine learning and facilitates quantitative analysis. Using 3P, we find that depletion of moesin, and to a lesser extent ezrin, decreases the proportion of cells with polarized MCAM. Furthermore, although co-polarized MCAM and ERM proteins show high spatial overlap, 3P identifies subpopulations with ERM proteins closer to the cell periphery. Live-cell imaging confirms that MCAM and ERM protein polarization is tightly coordinated, but ERM proteins enrich at the cell edge first. Finally, deletion of a juxtamembrane segment in MCAM previously shown to promote ERM protein interactions impedes MCAM polarization. Our findings highlight the requirement for ERM proteins in recruitment of MCAM to WRAMP structures and an advanced computational tool to characterize protein polarization.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:41:34.781.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047722
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Ebmeier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 10:25:43	ID requested
1	2024-05-22 12:19:37	announced
⏵ 2	2024-10-22 06:41:35	announced	2024-10-22: Updated project metadata.

10.6019/PXD047722;

10.1091/mbc.e23-06-0255;

Miller SG, Hoh M, Ebmeier CC, Tay JW, Ahn NG, Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma. Mol Biol Cell, 35(3):ar31(2024) [pubmed]

submitter keyword: Wnt5a, WRAMP,MCAM, ERM

Natalie G Ahn
contact affiliation	Distinguished Professor Associate Chair for Undergraduate Affairs Department of Biochemistry University of Colorado Boulder
contact email	natalie.ahn@colorado.edu
lab head
Christopher Ebmeier
contact affiliation	University of Colorado Boulder
contact email	christopher.ebmeier@colorado.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047722

PRIDE project URI

• PRIDE
  1. PXD047722
     1. Label: PRIDE project
     2. Name: Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma