PXD047711

PXD047711 is an original dataset announced via ProteomeXchange.

Title	Oleate grown bioreactor sludge metaproteome 2
Description	A combination of shotgun metaproteomics and 16S rRNA gene pyrosequencing wasused to identify potential functional pathways and key microorganisms involved in long-chain fatty acids (LCFA) anaerobic conversion. Microbial communities degrading saturated- and unsaturated-LCFA were compared. Archaeal communities were mainly composed of Methanosaeta, Methanobacterium and Methanospirillum species, both in stearate (saturated C18:0) and oleate (mono-unsaturated C18:1) incubations. Over 80% of the 16S rRNA gene sequences clustered within the Methanosaeta genus, which is in agreement with the high number of proteins assigned to this group (94%). Archaeal proteins related with methane metabolism were highly expressed. Bacterial communities were rather diverse and the composition dissimilar between incubations with saturated- and unsaturated-LCFA. Stearate-degrading communities were enriched in Deltaproteobacteria (34% of the assigned sequences), while microorganisms clustering within the Synergistia class were more predominant in oleate incubation (25% of the assigned sequences). Bacterial communities were diverse and active, given by the high percentage of proteins related with mechanisms of energy production. Several proteins were assigned to syntrophic bacteria, emphasizing the importance of the interactions between acetogens and methanogens in energy exchange and formation in anaerobic LCFA-rich environments.
HostingRepository	PRIDE
AnnounceDate	2024-05-03
AnnouncementXML	Submission_2024-05-03_08:40:08.231.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047711
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	João Sequeira
SpeciesList	scientific name: bioreactor sludge metagenome; NCBI TaxID: 412754; scientific name: cellular organisms; NCBI TaxID: NCBITaxon:131567;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Dionex instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 07:26:56	ID requested
⏵ 1	2024-05-03 08:40:09	announced

10.6019/PXD047711;

submitter keyword: bioreactor sludge metaproteome, fatty acids degradation, oleate

Joao Carlos Sequeira
contact affiliation	Centre of Biological Engineering, Minho University
contact email	jsequeira@ceb.uminho.pt
lab head
João Sequeira
contact affiliation	Centre of Biological Engineering
contact email	maildosequeira@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047711
     1. Label: PRIDE project
     2. Name: Oleate grown bioreactor sludge metaproteome 2