PXD047705

PXD047705 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteins of anti-sigma70 and anti-RNAP immunoprecipitates in bacteria
Description	As part of the project aimed at the interaction of RNA with RNA polymerase and primary sigma factor in bacteria we analyzed anti-σ70 antibody immunoprecipitates from Mycobacterium smegmatis stationary phase to identify proteins in σA complex in non-treated cells. We performed mass spectrometry analysis of anti-σ70 antibody and anti-RNAP immunoprecipitates from Corynebacterium glutamicum stationary phase to identify proteins present in σA and RNAP complexes.
HostingRepository	PRIDE
AnnounceDate	2024-03-06
AnnouncementXML	Submission_2024-03-06_00:03:39.557.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petr Halada
SpeciesList	scientific name: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155); NCBI TaxID: 246196; scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF SCP

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-11 03:37:36	ID requested
⏵ 1	2024-03-06 00:03:40	announced

Publication List

Hausnerov, á VV, Shoman M, Kumar D, Schwarz M, Modr, á, k M, Mat, ě, j, č, kov, á JJ, Mikeskov, á E, Neva S, Herrmannov, á A, Š, ikov, á M, Halada P, Novotn, á I, Pajer P, Val, á, š, ek LS, P, ř, evorovsk, ý M, Kr, á, sn, ý L, Hnilicov, á J, RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria. Nucleic Acids Res, ():(2024) [pubmed]
10.1093/nar/gkae081;

Hausnerov, á VV, Shoman M, Kumar D, Schwarz M, Modr, á, k M, Mat, ě, j, č, kov, á JJ, Mikeskov, á E, Neva S, Herrmannov, á A, Š, ikov, á M, Halada P, Novotn, á I, Pajer P, Val, á, š, ek LS, P, ř, evorovsk, ý M, Kr, á, sn, ý L, Hnilicov, á J, RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria. Nucleic Acids Res, ():(2024) [pubmed]

10.1093/nar/gkae081;

Keyword List

submitter keyword: sigma A, RNAP, transcription, Corynebacterium glutamicum, Mycobacterium smegmatis

submitter keyword: sigma A, RNAP, transcription, Corynebacterium glutamicum, Mycobacterium smegmatis

Contact List

Petr Novak
contact affiliation	Laboratory of Structural Biology and Cell Signaling Institute of Microbiology of the CAS Division BIOCEV Průmyslová 595 252 50 Vestec Czech Republic
contact email	pnovak@biomed.cas.cz
lab head
Petr Halada
contact affiliation	Institute of Microbiology, The Czech Academy of Sciences, Prague 14220, Czech Republic
contact email	halada@biomed.cas.cz
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD047705
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD047705

PRIDE project URI

Repository Record List

[ + ]