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PXD047697

PXD047697 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title0549_2 Fission yeast Pab1 interactome
DescriptionWe performed mass-spectrometry analysis of proteins co-purifying with the Pab1, the B55 regulatory subunit of the PP2A protein phosphatase complex. Pab1 was tagged with YFP at the C-terminus and expressed from its endogenous promoter at its chromosomal locus. After one hour of nitrogen starvation, Pab1-YFP was pulled down and co-purifying proteins were analysed by mass-spectrometry. Our results revealed the presence of all components of PP2A protein complexes including Paa1, the catalytic subunits Ppa1, Ppa2, and Ppa3.
HostingRepositoryPRIDE
AnnounceDate2024-12-02
AnnouncementXMLSubmission_2024-12-02_00:53:30.181.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSergio Moreno
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690; scientific name: Schizosaccharomyces pombe; NCBI TaxID: NCBITaxon:4896;
ModificationListiodoacetamide derivatized residue
InstrumentBruker Daltonics timsTOF series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-11 00:57:49ID requested
12024-12-02 00:53:30announced
Publication List
10.1038/S41467-024-55004-4;
Keyword List
submitter keyword: Fission yeast, PP2A, Pab1-YFP interactome
Contact List
Sergio Moreno
contact affiliationInstituto de Biología Funcional y Genómica. CSIC / Salamanca University -Spain-
contact emailsmo@usal.es
lab head
Sergio Moreno
contact affiliationInstituto de Biología Funcional y Genómica. CSIC / Salamanca University
contact emailsmo@usal.es
dataset submitter
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