PXD047691
PXD047691 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Target identification of dioxygenated amide derivatives interacting proteins |
Description | To identify the direct binding proteins of dioxygenated amide by chemical proteomics, we firstly evaluated the efficiency of optimized probe 6D-PP probe labelling the E. coli bacterial proteomes by in-gel fluorescence. Briefly, the E. coli bacterial lysates were incubated with 100 M probe 6D-PP under 37 °C for 2 h and exposed to 365 nm UV light for 0.5 h to initiate photo-crosslinking. A blank control with only DMSO, a negative control without UV light and a control sample with pretreatment of 500 M D-PP6-C were also included. After conjugation with the TAMRA-N3 by copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry for another 1 h, the probe 6D-PP labelled proteins were separated with 12% SDS-PAGE and imaged by in-gel fluorescence (Fig.3B). The probe 6D-PP probe showed an efficiently UV-dependent and probe 6D-PP specific labelling, supporting it’s readily applicable in ABPP (activity-based protein profiling) experiment. |
HostingRepository | iProX |
AnnounceDate | 2023-12-08 |
AnnouncementXML | Submission_2024-02-03_20:56:03.758.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-12-10 20:19:16 | ID requested | |
1 | 2023-12-10 20:19:36 | announced | |
⏵ 2 | 2024-02-03 20:56:04 | announced | 2024-02-04: Update information. |
Publication List
Chang H, Ji R, Zhu Z, Wang Y, Yan S, He D, Jia Q, Huang P, Cheng T, Wang R, Zhou Y, Target identification, and optimization of dioxygenated amide derivatives as potent antibacterial agents with FabH inhibitory activity. Eur J Med Chem, 265():116064(2024) [pubmed] |
Keyword List
submitter keyword: antibacterial agents, FabH inhibitory activity |
Contact List
Rui Wang | |
---|---|
contact affiliation | Shenzhen Bay Laboratory |
contact email | wangrui@szbl.ac.cn |
lab head | |
Rui Wang | |
contact affiliation | Shenzhen Bay Laboratory |
contact email | wangrui@szbl.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |