PXD047683

PXD047683 is an original dataset announced via ProteomeXchange.

Title	Microenvironment Governed Photolysis Reaction for Suborganelle Protein Proximity Labeling
Description	Photolysis reactions are designed to preferentially occur in polar aqueous environment to accommodate biological applications. Described herein is a collection of aminobenzoquinone based photocages with accelerated photolytic kinetics in non-polar conditions. Structural investigation demonstrates the role of cyclic ring size, steric substituent, and electronic effect in regulating the reaction kinetics (KH2O & Kdioxane) and polarity preference (Kdioxane/KH2O). Rational introduction of optimal moieties leads to up to 5.2-fold kinetic acceleration (61.4×10-2 min-1) and 21.7-fold non-polar selectivity. Photochemical spectroscopic characterizations and theoretical calculations together explain the rationales underlying structure-polarity relationship. Notably, the uncaged ortho-quinone methide product bears covalent reactivity to-wards diverse nucleophiles of a protein, primarily Cys (66.7%), revealed by tandem mass spectrometry. Eventually, such lipophilic photolysis reaction is applied to selective capture of proteome in proximity to lipid droplets (LDs) in human diseased liver tissues. Downstream proteomic profiling of labeled proteins uncovers inter-organellar interactions with LDs. This work exemplifies photolysis reactions can act on-demand by biological microenvironment in addition to optical regulations.
HostingRepository	iProX
AnnounceDate	2023-12-08
AnnouncementXML	Submission_2023-12-10_18:23:38.782.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ci Wu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-10 18:23:06	ID requested
⏵ 1	2023-12-10 18:23:39	announced

Dataset with its publication pending

submitter keyword: photocage, photolysis, proximity labeling, quinone methide, lipid droplets

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
Ci Wu
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	wuci1111@126.com
dataset submitter

iProX dataset URI