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PXD047676

PXD047676 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics of dried blood collected on Mitra devices
DescriptionVenous blood was collected in Li-heparin or EDTA blood tubes and loaded onto 20 uL Mitra devices or separated to plasma as detailed in metadata. Samples were denatured in 5.5% SDC, reduced and alkylated, and digested with TPCK-trypsin followed by acid precipitation. 1 mg of filtered precipitates were enriched for phosphopeptides using TiO. Data was acquied using nanoLC, Evosep One or SPE-ZipChip. Data was acquired by DIA (Exploris 480 or TimsTOF Pro 2) or LFQ-DDA. DIA data was analyzed with Spectronaut 18 and DDA with Skyline.
HostingRepositoryMassIVE
AnnounceDate2023-12-10
AnnouncementXMLSubmission_2023-12-10_21:41:11.890.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatt Foster
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Phospho
InstrumentOrbitrap Exploris 480; Orbitrap Exploris 240; timsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-10 07:42:10ID requested
12023-12-10 21:41:12announced
Publication List
no publication
Keyword List
submitter keyword: phosphorylation, data-independent acquisition
Contact List
Matthew W. Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
lab head
Matt Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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