PXD047672

PXD047672 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Novel mechanisms of 5-Fluorouracil Resistance in Colorectal Cancer: A Proteomic Investigation
Description	One of the main obstacles to therapeutic success in colorectal cancer (CRC) is the development of acquired resistance to treatment with drugs such as 5-fluorouracil (5-FU), the most commonly used drug in CRC patients. Whilst some mechanisms of resistance are well-known, it is clear from the stasis in therapy success rate, that much is still unknown. Here a proteomics approach is taken towards identification using 5-FU resistant sublines of human CRC cell lines generated in-house. Using a multiplexed stable isotope labelling with amino acids in cell culture (SILAC) proteomics strategy, the resistant cell lines, and equivalently passaged 5-FU-sensitive cell lines were compared to parent cell lines grown in Heavy medium using 2D liquid chromatography and Orbitrap Fusion™ Tribrid™ Mass Spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-16_04:43:30.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sadr Shaheed
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-09 18:14:30	ID requested
⏵ 1	2024-06-16 04:43:31	announced

Publication List

10.3390/cells13040342;
Ortega Duran M, Shaheed SU, Sutton CW, Shnyder SD, A Proteomic Investigation to Discover Candidate Proteins Involved in Novel Mechanisms of 5-Fluorouracil Resistance in Colorectal Cancer. Cells, 13(4):(2024) [pubmed]

10.3390/cells13040342;

Ortega Duran M, Shaheed SU, Sutton CW, Shnyder SD, A Proteomic Investigation to Discover Candidate Proteins Involved in Novel Mechanisms of 5-Fluorouracil Resistance in Colorectal Cancer. Cells, 13(4):(2024) [pubmed]

Keyword List

submitter keyword: colorectal cancer
drug resistance mechanisms
in vitro models
proteomics
5-fluorouracil
stable isotope labelling with amino acids in cell culture (SILAC)

submitter keyword: colorectal cancer

drug resistance mechanisms

in vitro models

proteomics

5-fluorouracil

stable isotope labelling with amino acids in cell culture (SILAC)

Contact List

Dr Sadr ul Shaheed
contact affiliation	Nuffield department of Surgical Sciences, University of Oxford UK
contact email	sadr.shaheed@nds.ox.ac.uk
lab head
Sadr Shaheed
contact affiliation	Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom
contact email	sadr.shaheed@nds.ox.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD047672
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD047672

PRIDE project URI

Repository Record List

[ + ]