PXD047671

PXD047671 is an original dataset announced via ProteomeXchange.

Title	Unveiling Protein Structure under Pressure on the Proteome Scale
Description	Investigating protein structure, conformation, and activity under hydrostatic pressure is a critical challenge, particularly since a sizable portion of life is marine or deep subsurface. There is a perception that most proteins experience limited changes under pressures environmentally relevant on Earth; this conclusion is biased as studies focus on pressure sensitivity within a select few individual molecules, which tend to be “well-behaved”, stable proteins. More high-throughput biophysical techniques must be employed to identify promising model systems with measurable pressure effects to expand the investigation for piezo-tolerance and -sensitivity origins. Limited proteolysis (LiP) is a technique that uses differential non-specific protease susceptibility to gauge protein structure and conformational changes. When combined with mass spectrometry (MS), this method permits the profiling of subtle structural alterations across entire proteomes. The impressive throughput of LiP-MS offers a unique opportunity to survey hydrostatic pressure effects en masse. Here, we introduce the first high-pressure structural proteomics study with novel, purpose-built equipment allowing LiP-MS to be completed under ocean-bottom pressures (100 MPa). Results in a piezo-sensitive model species, Thermus thermophilus¸ show whole-proteome structural changes are rapid and more extensive than anticipated, demonstrating the capacity of high-pressure LiP-MS (HiP-LiP-MS) to profile pressure-sensitive proteins and provide a rich source of new targets for future studies.
HostingRepository	PRIDE
AnnounceDate	2024-09-12
AnnouncementXML	Submission_2024-09-12_14:10:58.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haley Moran
SpeciesList	scientific name: Thermus thermophilus HB27; NCBI TaxID: 262724;
ModificationList	acetylated residue; monohydroxylated residue; formylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-09 18:06:09	ID requested
⏵ 1	2024-09-12 14:10:59	announced

10.1103/PRXLIFE.2.033011;

submitter keyword: High Pressure, Thermus thermophilus,Limited Proteolysis, Pulse Proteolysis, Bottom Up Proteomics, Extremophiles, Structural Proteomics

Stephen Fried
contact affiliation	Johns Hopkins University Department of Chemistry
contact email	sdfried@jhu.edu
lab head
Haley Moran
contact affiliation	Johns Hopkins University
contact email	hmoran2@jh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047671
     1. Label: PRIDE project
     2. Name: Unveiling Protein Structure under Pressure on the Proteome Scale