PXD047669
PXD047669 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multi-omics analysis of aggregative multicellularity |
| Description | The extent to which mRNA and protein levels correlate is still not fully known, especially during development, when cells undergo a major transition in gene expression. One organism with particular development is Dictyostelium discoideum, during which it transitions from free-living unicellular to multicellular. Previously the transcriptome has been thoroughly studied during multicellular development, however the proteome and its correlation to the transcriptome during this transition is not fully understood. In this study, for the first time, paired transcriptomics and proteomics was performed in a time series during aggregative multicellularity. From the analysis, the majority of transcripts were identified as differentially expressed, and we could quantify roughly a third of the proteome during early multicellular development. The proteome and transcriptome correlate relatively highly during steady-state, however this decreases as soon as multicellular aggregation is initiated. Correlation is particularly low at the gene-level during development. We find that dynamically regulated mRNA often leads to linear up- or downregulation of the protein, and that there is a time lag of approximately 2 to 4 hours between mRNA and protein. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-03-08 |
| AnnouncementXML | Submission_2024-03-08_05:49:27.818.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Bart Edelbroek |
| SpeciesList | scientific name: Dictyostelium discoideum; NCBI TaxID: 44689; |
| ModificationList | Oxidation; GG; Carbamidomethyl; Acetyl |
| Instrument | Q Exactive GC Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-12-09 12:05:04 | ID requested | |
| ⏵ 1 | 2024-03-08 05:49:28 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Multi-omics, Transcriptomics, Proteomics, mRNA-protein correlation, Aggregative multicellularity, Amoebozoa, Dictyostelium discoideum |
Contact List
| Fredrik Soderbom | |
|---|---|
| contact affiliation | Uppsala University |
| contact email | fredrik.soderbom@icm.uu.se |
| lab head | |
| Bart Edelbroek | |
| contact affiliation | Uppsala University |
| contact email | bart.edelbroek@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093620/ |
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