PXD047660
PXD047660 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of complex III, NQR and SDH as pharmacologic targets in non-multiplying Pseudomonas aeruginosa cultured in urine-like conditions |
| Description | Bacterial membrane samples (20 ug of protein) were prepared using the Filter Aided Sample Preparation protocol, employing 30 K NMWL centrifugal filter units. Samples were reduced with 20 mM dithiothreitol, followed by 20 min alkylation with 50 mM iodoacetamide. The alkylated proteins underwent three washes with 200 uL of 8 M Urea in 0.1 M Ammonium Bicarbonate (ABC) and were subsequently equilibrated three times with 0.1 M ABC prior to trypsin digestion. Proteins were digested using Trypsin at an enzyme: protein ratio of 1:50 in 50 uL of 0.1 M ABC buffer at 37C overnight. The resulting digested peptides were collected by centrifugation at 14,000 g for 20 min, followed by two elutions with 50 uL of 50 mM ABC and one elution with 50 uL of 0.5 M NaCl. The eluted peptides were desalted using C18 columns and were dried. The digested proteins were then reconstituted in a 30 uL solution of 5% acetonitrile and 0.1% formic acid buffer for LC-MS analysis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-01-15 |
| AnnouncementXML | Submission_2024-01-15_09:14:48.200.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Oscar Juarez |
| SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-12-08 11:09:02 | ID requested | |
| ⏵ 1 | 2024-01-15 09:14:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Pseudomonas aeruginosa, aerobic metabolism, respiratory chain, NQR, bc1 complex, SDH, urinary tract infection, metabolic activation, target identification |
Contact List
| Oscar Juarez | |
|---|---|
| contact affiliation | Illinois Institute of Technology |
| contact email | ojuarez@iit.edu |
| lab head | |
| Oscar Juarez | |
| contact affiliation | Illinois Institute of Technology |
| contact email | ojuarez@iit.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000093610/ |
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