PXD047647

PXD047647 is an original dataset announced via ProteomeXchange.

Title	Characterization and proteomic analysis of extracellular vesicles recovered from serum of healthy and dogs naturally infected by Babesia canis
Description	Canine babesiosis, a tick-borne disease, is characterized by protozoan parasites invading red blood cells. It is rapidly expanding in many European countries. Examining extracellular vesicles (EVs) and their protein cargo has the potential to offer crucial insights into the response to Babesia canis infection, presenting opportunities for advancements in veterinary diagnostic and therapeutic strategies. In the present study, we have a) isolated small EVs (< 200 nm) from the serum of 15 healthy dogs and 15 dogs naturally infected with B. canis using size-exclusion chromatography (fraction 2 and 3 per each sample), (2) characterized isolated EVs by nanoparticle tracking analysis, transmission electron microscopy and Western blot (3) analysed the protein cargo of isolated EVs by mass spectrometry. We hypothesized that there will be a difference in EV characteristics (size, concentration, EV marker proteins) and profiles of luminal proteins between the two experimental groups. Our aim was to characterize proteins that can offer valuable insights into B. canis infection in dogs, thereby unravelling the complex mechanisms of B. canis infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:03.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dina Rešetar Maslov
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-08 04:25:49	ID requested
1	2024-04-23 04:29:50	announced
⏵ 2	2024-10-22 06:37:03	announced	2024-10-22: Updated project metadata.

10.1016/J.VETPAR.2024.110188;

submitter keyword: canine babesiosis, proteomics, extracellular vesicles, Babesia canis,dog

Vladimir Mrljak
contact affiliation	Laboratory of proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova street 55, 10000 Zagreb, Croatia
contact email	vmrljak@vef.unizg.hr
lab head
Dina Rešetar Maslov
contact affiliation	Faculty of Veterinary Medicine of University of Zagreb
contact email	drmaslov@vef.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD047647

PRIDE project URI

• PRIDE
  1. PXD047647
     1. Label: PRIDE project
     2. Name: Characterization and proteomic analysis of extracellular vesicles recovered from serum of healthy and dogs naturally infected by Babesia canis