PXD047645

PXD047645 is an original dataset announced via ProteomeXchange.

Title	GPRC Function in autophagy control: a systematic approach of chemical intervention
Description	Autophagy facilitates the degradation of cellular content via the lysosome and is involved in cellular homeostasis and stress response pathways. As such, malfunction of autophagy is linked to a variety of diseases ranging from organ specific illnesses like cardiomyopathy to systemic illnesses such as cancer or metabolic syndromes. Given the variety of autophagic functions within a cell and tissue, regulation of autophagy is complex and contains numerous positive and negative feedback loops. While our knowledge of mechanisms for cargo selectivity has significantly improved over the last decade, our understanding of signaling routes activating individual autophagy pathways remains rather sparce. In this resource study, we report on a well-characterized chemical library assembled within the IMI initiative EUbOPEN containing 77 GPCR-targeting ligands that was used to systematically analyze LC3B-based autophagy as well as ER-phagy flux upon compound treatment. The global impact of hit compounds TC-G 1004, BAY 60-6583, PSNCBAM-1, TC-G 1008, LPA2 Antagonist 1, ML154, JTC-801 and ML-290 targeting Adenosine receptor A2a (ADORA2A), Adenosine receptor A2b (ADORA2B), Cannabinoid receptor 1 (CNR1), G-protein coupled receptor 39 (GPR39), Lysophosphatidic acid receptor 2 (LPAR2), neuropeptide S receptor 1 (NPSR1), NOP (OPRL1), and Relaxin receptor 1 (RXFP1) were subsequently analyzed and compared by TMT-based mass spectrometry. Our results indicate the differential impact of targeted GPCRs on known autophagy-associated proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:50:47.250.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pablo Sanz Martinez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-08 03:51:45	ID requested
1	2024-07-18 10:09:01	announced
⏵ 2	2024-10-22 06:50:47	announced	2024-10-22: Updated project metadata.

10.1016/J.JMB.2024.168643;

submitter keyword: Human, Multiplexed, LC-MS,TMTpro 18plex,GPRC,Compounds

Dr.Alexandra Stolz
contact affiliation	Institute for biochemistry II, ER quality control
contact email	stolz@em.uni-frankfurt.de
lab head
Pablo Sanz Martinez
contact affiliation	PhD student
contact email	P.SanzMartinez@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD047645

PRIDE project URI

• PRIDE
  1. PXD047645
     1. Label: PRIDE project
     2. Name: GPRC Function in autophagy control: a systematic approach of chemical intervention