PXD047620

PXD047620 is an original dataset announced via ProteomeXchange.

Title	Thermothielavioides terrestris secretome after growth on raw and mildly steam-pretreated spruce
Description	One important obstacle in using softwood in biorefineries is its recalcitrance to enzymatic saccharification. In the present work, Thermothielavioides terrestris LPH172 was cultivated on three different steam-pretreated spruce materials and on non-treated spruce, harboring different hemicellulose content and structural characteristics. The aim of the work was to map the substrate-induced changes in the secretome of T. terrestris when grown on differently treated spruce materials and evaluate the hydrolytic efficiency of the secretome as supplement of a commercial enzyme mixture. The cultivation of T. terrestris was monitored by enzyme activity measurements of endo-cellulase, endo-xylanase, endo-mannanase, laccase and peroxidase. Proteomic analysis was performed on the secretomes induced by these materials to map the differences in enzyme expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_05:07:19.792.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047620
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Romanos Siaperas
SpeciesList	scientific name: Thermothielavioides terrestris; NCBI TaxID: 2587410;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-07 09:01:43	ID requested
⏵ 1	2024-10-11 05:07:20	announced

10.6019/PXD047620;

10.1186/s13068-024-02569-3;

Caputo F, Siaperas R, Dias C, Nikolaivits E, Olsson L, Elucidating Thermothielavioides terrestris secretome changes for improved saccharification of mild steam-pretreated spruce. Biotechnol Biofuels Bioprod, 17(1):127(2024) [pubmed]

submitter keyword: CAZy, TMT, spruce,Thermothielavioides terrestris, Orbitrap Fusion Lumos

Lisbeth Olsson
contact affiliation	Wallenberg Wood Science Centre, Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden
contact email	lisbeth.olsson@chalmers.se
lab head
Romanos Siaperas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	roman_siap@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD047620

PRIDE project URI

• PRIDE
  1. PXD047620
     1. Label: PRIDE project
     2. Name: Thermothielavioides terrestris secretome after growth on raw and mildly steam-pretreated spruce