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PXD047620

PXD047620 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThermothielavioides terrestris secretome after growth on raw and mildly steam-pretreated spruce
DescriptionOne important obstacle in using softwood in biorefineries is its recalcitrance to enzymatic saccharification. In the present work, Thermothielavioides terrestris LPH172 was cultivated on three different steam-pretreated spruce materials and on non-treated spruce, harboring different hemicellulose content and structural characteristics. The aim of the work was to map the substrate-induced changes in the secretome of T. terrestris when grown on differently treated spruce materials and evaluate the hydrolytic efficiency of the secretome as supplement of a commercial enzyme mixture. The cultivation of T. terrestris was monitored by enzyme activity measurements of endo-cellulase, endo-xylanase, endo-mannanase, laccase and peroxidase. Proteomic analysis was performed on the secretomes induced by these materials to map the differences in enzyme expression.
HostingRepositoryPRIDE
AnnounceDate2024-10-11
AnnouncementXMLSubmission_2024-10-11_05:07:19.792.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD047620
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRomanos Siaperas
SpeciesList scientific name: Thermothielavioides terrestris; NCBI TaxID: 2587410;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-07 09:01:43ID requested
12024-10-11 05:07:20announced
Publication List
10.6019/PXD047620;
10.1186/s13068-024-02569-3;
Caputo F, Siaperas R, Dias C, Nikolaivits E, Olsson L, Elucidating Thermothielavioides terrestris secretome changes for improved saccharification of mild steam-pretreated spruce. Biotechnol Biofuels Bioprod, 17(1):127(2024) [pubmed]
Keyword List
submitter keyword: CAZy, TMT, spruce,Thermothielavioides terrestris, Orbitrap Fusion Lumos
Contact List
Lisbeth Olsson
contact affiliationWallenberg Wood Science Centre, Department of Biology and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden Division of Industrial Biotechnology, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden
contact emaillisbeth.olsson@chalmers.se
lab head
Romanos Siaperas
contact affiliationIndustrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact emailroman_siap@hotmail.com
dataset submitter
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Dataset FTP location
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