PXD047619

PXD047619 is an original dataset announced via ProteomeXchange.

Title	From the fat body to the hemolymph: Profiling tick immune and storage proteins through transcriptomics and proteomics
Description	Ticks are blood-feeding arachnids that are known to transmit various pathogenic microorganisms to their hosts. During blood feeding, ticks activate their metabolism and immune system to efficiently utilise nutrients from the host's blood and complete the feeding process. In contrast to insects, in which the fat body is known to be a central organ that controls essential metabolic processes and immune defence mechanisms, the function of the fat body in tick physiology is still relatively unexplored. To fill this gap, we sought to uncover the repertoire of genes expressed in the fat body associated with trachea (FB/Tr) by analyzing the transcriptome of individual, partially fed (previtellogenic) Ixodes ricinus females . The resulting catalog of individual mRNA sequences reveals a broad repertoire of transcripts encoding proteins involved in nutrient storage and distribution, as well as components of the tick immune system. To gain a detailed insight into the secretory products of FB/Tr specifically involved in inter-tissue transport and humoral immunity, the transcriptomic data were complemented with the proteome of soluble proteins in the hemolymph of partially fed female ticks. Among these proteins, the hemolipoglyco-carrier proteins were predominant. When comparing immune peptides and proteins from the fat body with those produced by hemocytes, we found that the fat body serves as a unique producer of certain immune components. Finally, time-resolved transcriptional regulation of selected immune transcripts from the FB/Tr was examined in response to experimental challenges with model microbes and analysed by RT-qPCR. Overall, our data show that the fat body of ticks, similar to insects, is an important metabolic tissue that also plays a remarkable role in immune defense against invading microbes. These findings improve our understanding of tick biology and its impact on the transmission of tick-borne pathogens.
HostingRepository	PRIDE
AnnounceDate	2024-01-11
AnnouncementXML	Submission_2024-01-11_05:43:09.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Filip Dyčka
SpeciesList	scientific name: Ixodes ricinus; NCBI TaxID: 34613;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-07 09:00:49	ID requested
⏵ 1	2024-01-11 05:43:09	announced

10.1016/J.IBMB.2024.104072;

submitter keyword: LC-MSMS, proteomics, hemolymph, tick, Ixodes ricinus,Fat body

Petr Kopáček
contact affiliation	Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 1160/31, 37005 Ceske Budejovice, Czech Republic
contact email	kopajz@paru.cas.cz
lab head
Filip Dyčka
contact affiliation	University of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact email	dycka@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD047619

PRIDE project URI

• PRIDE
  1. PXD047619
     1. Label: PRIDE project
     2. Name: From the fat body to the hemolymph: Profiling tick immune and storage proteins through transcriptomics and proteomics