PXD047610

PXD047610 is an original dataset announced via ProteomeXchange.

Title	Lectin-based SP3 technology enables N-glycoproteomics analysis
Description	We developed a glycosylation enrichment method based on lectin and single-pot, solid-phase-enhanced sample preparation (SP3) technology, termed lectin-based SP3 technology (LectinSP3). LectinSP3 immobilized lectin on the SP3 beads for N-glycopeptide enrichment. It could identify over 1,100 N-glycosylation sites and 600 N-glycoproteins from 10 μg of mouse testis peptides. Furthermore, using LectinSP3 method, we characterized the N-glycoproteome of 1,000 mouse oocytes in three replicates, and identified a total of 363 N-glycosylation sites from 215 N-glycoproteins.
HostingRepository	PRIDE
AnnounceDate	2024-04-10
AnnouncementXML	Submission_2024-04-10_07:57:27.411.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yueshuai Guo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-07 05:12:34	ID requested
⏵ 1	2024-04-10 07:57:27	announced

Dataset with its publication pending

submitter keyword: testis, glycoproteomics, SP3, oocyte,N-glycosylation, lectin

Xuejiang Guo
contact affiliation	State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing 211166, China
contact email	guo_xuejiang@njmu.edu.cn
lab head
Yueshuai Guo
contact affiliation	Nanjing Medical University
contact email	gys_706@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047610
     1. Label: PRIDE project
     2. Name: Lectin-based SP3 technology enables N-glycoproteomics analysis