PXD047584

PXD047584 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomic AnalyComparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patientssis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patients
Description	The pathogenesis of myopia involves different molecular mechanisms and pathways, which can be reflected in the aqueous humor. In our study, aqueous humor was collected for liquid chromatograph mass spectrometer (LC/MS) analysis from 30 individual eyes that underwent phacoemulsification and intraocular lens (IOL) implantation. A total of 190 DEPs (differentially expressed proteins) were identified, which revealed their involvement in pathways such as carbon metabolism, lipid, and cholesterol metabolism, ECM (extracellular matrix) component regulation, and scleral remodeling in the context of high myopia. Further analysis revealed 15 interacting hub proteins, encompassing functions related to lipoprotein assembly, regulation, complement cascades, and fibrin formation. Subsequent validation demonstrated elevated levels of APOE (apolipoprotein E), C3 (complement 3), and AHSG (alpha-2-HS-glycoprotein) in the high myopia group (31 eyes of cataracts and 45 eyes of high myopia with cataracts), while AHSG had a significant positive correlation with axial length in patients with high myopia and good efficacy in distinguishing between myopic and non-myopic groups. These results suggested that the interaction of lipoproteins, complement cascades, and the fibrinolytic system played a pivotal role in the pathological process of myopia, while the metabolism of essential materials and the remodeling of ECM and tissue structure were affected in the eyes of individuals with high myopia.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:01.449.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingxiao Du
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-06 15:49:34	ID requested
1	2024-05-23 01:19:36	announced
⏵ 2	2024-10-22 06:42:02	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.3c00630;

Du J, Liu D, Zhou W, Ye T, Zhang C, Qian T, Zhang J, Zhang Z, Comparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in the Eyes of High Myopic Patients. J Proteome Res, 23(3):916-928(2024) [pubmed]

submitter keyword: lipoprotein, myopia, proteomic analysis,aqueous humor, axial length

Zhihua Zhang
contact affiliation	Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
contact email	drzhihuazhang@126.com
lab head
Jingxiao Du
contact affiliation	Shanghai General Hospital
contact email	d1084877951@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047584

PRIDE project URI

• PRIDE
  1. PXD047584
     1. Label: PRIDE project
     2. Name: Comparative Proteomic AnalyComparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patientssis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patients