PXD047581 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Shotgun proteomics analysis of nuclear and mitochondrial from human coronary artery endothelial cells fraction treated with calcioprotein particles |
Description | Calciprotein particles (CPPs) are indispensable scavengers of excessive Ca2+ and PO43- ions in the blood, being internalized and recycled by liver and spleen macrophages, monocytes, and endothelial cells (ECs). Despite the fact, that CPPs are associated with many cardiovascular diseases and are known to trigger endothelial dysfunction, the exact mechanisms of its recycling by ECs are still poorly studied. Particularly, because of the low resolution of the standard proteomics approach. We assume that CPPs internalization would cause oxidative stress and strong rearrangements in the transcription. Therefore, we performed a shotgun proteomics analysis of nuclear and mitochondrial fraction of human coronary artery endothelial cells (HCAEC) treated with primary (amorphous) or secondary (crystalline) CPPs (CPP-P and CPPs, respectively). Contact: Dr Anton G. Kutikhin, Laboratory of Molecular, Translational and Digital Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russia (lab head). |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-31 |
AnnouncementXML | Submission_2023-12-30_22:11:17.811.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Egor Repkin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-06 11:50:20 | ID requested | |
⏵ 1 | 2023-12-30 22:11:18 | announced | |
Publication List
Keyword List
submitter keyword: Calciprotein particles, TimsToF Pro, DDA-PASEF, mitochondria, endothelium, nucleus, subcellular fractionation |
Contact List
Dr. Anton G. Kutikhin |
contact affiliation | Laboratory of Molecular, Translational and Digital Medicine, Research Institute for Complex Issues of Cardiovascular Diseases, Kemerovo, Russia |
contact email | antonkutikhin@gmail.com |
lab head | |
Egor Repkin |
contact affiliation | Saint-Petersburg State University |
contact email | erepkin53@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047581 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047581
- Label: PRIDE project
- Name: Shotgun proteomics analysis of nuclear and mitochondrial from human coronary artery endothelial cells fraction treated with calcioprotein particles