PXD047578

PXD047578 is an original dataset announced via ProteomeXchange.

Title	Phenotypic and Proteomic Insights into Differential Cadmium Accumulation in Maize Kenerls
Description	The contamination of agricultural soil by heavy metal cadmium (Cd) poses a significant environmental challenge, affecting crop growth, development and, human health. Previous studies have established the pivotal role of the ZmHMA3 gene, a P-type ATPase heavy metal transporter, in determining variable Cd accumulation in maize grains among 513 inbred lines. To decipher the molecular mechanism underlying mutation-induced phenotypic differences mediated by ZmHMA3, we conducted a quantitative Tandem Mass Tag (TMT)-based proteomic analysis of immature maize kernels. This analysis aimed to identify differentially expressed proteins (DEPs) in wild-type B73 and zmhma3 null mutant under Cd stress. The findings demonstrated that zhma3 accumulated higher levels of Cd compared to B73 when exposed to varying Cd concentrations in the soil. In comparison to low Cd concentration soil, B73 and zmhma3 exhibited 75 and 142 DEPs, respectively, with 24 common DEPs shared between them. Zmhma3 showed a higher induction of upregulated genes related to Cd stres than B73. Amino sugar and nucleotide sugar metabolism were specifically enriched in B73, while phenylpropanoid biosynthesis, nitrogen metabolism, and glyoxylate and dicarboxylate metabolism appeared to play a more significant role in zmhma3. This study provides proteomics insights into unraveling the molecular mechanism underlying the differences in Cd accumulation in maize kernel.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_10:22:28.146.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Su Tian
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-06 08:59:45	ID requested
⏵ 1	2024-01-26 10:22:28	announced

10.3390/genes14122204;

Guo H, Deng M, Yu F, Li H, Cao Z, Zeng Q, Chen Z, Luo H, Tang B, Phenotypic and Proteomic Insights into Differential Cadmium Accumulation in Maize Kernels. Genes (Basel), 14(12):(2023) [pubmed]

submitter keyword: maize

cadmium

proteomics

kernel

mutant

Bin Tang
contact affiliation	Hunan Academy of Agricultural Sciences
contact email	tangbin@hunaas.cn
lab head
Su Tian
contact affiliation	xiangya hospital
contact email	251906072@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD047578

PRIDE project URI

• PRIDE
  1. PXD047578
     1. Label: PRIDE project
     2. Name: Phenotypic and Proteomic Insights into Differential Cadmium Accumulation in Maize Kenerls