PXD047550

PXD047550 is an original dataset announced via ProteomeXchange.

Title	Brain region differences in α1 and α5 subunit-containing GABAA receptor proteome revealed by affinity purification and Blue Native PAGE proteomics
Description	GABAA receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β and one γ or δ subunit. From the six α subunit genes, the α5 subunit displays a limited spatial expression pattern and is known to mediate both phasic and tonic inhibition. In this study, using immunoaffinity-based proteomics we identified the α5 subunit containing receptor complexes in hippocampus and olfactory bulb. The α1-α5 interaction was identified in both brain regions albeit with significantly different stoichiometries. In line with this, reverse IPs using anti-α1 antibody showed the α5-α1 co-occurrence and validated the quantitative difference. In addition, we showed that the association of Neuroligin 2 with α1-containing receptors was much higher in olfactory bulb than hippocampus, which was confirmed using blue native gel electrophoresis and quantitative mass spectrometry. Finally, immunocytochemical staining revealed co-localization of α1 and α5 subunits in post-synaptic puncta in the hippocampus.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:29:03.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-05 18:20:54	ID requested
⏵ 1	2024-01-26 07:29:04	announced

10.3390/cells13010014;

Chen M, Koopmans F, Gonzalez-Lozano MA, Smit AB, Li KW, Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics. Cells, 13(1):(2023) [pubmed]

submitter keyword: GABAAR, proteomics, mass spectrometry, immunoprecipitation

Kawan Li
contact affiliation	CNCR,VU Amsterdam
contact email	k.w.li@vu.nl
lab head
Miao Chen
contact affiliation	VU Amsterdam
contact email	smores1990@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047550
     1. Label: PRIDE project
     2. Name: Brain region differences in α1 and α5 subunit-containing GABAA receptor proteome revealed by affinity purification and Blue Native PAGE proteomics