PXD047544

PXD047544 is an original dataset announced via ProteomeXchange.

Title	Ribosomal protein S27A pseudogene 5 is involved in monosome related RNA translation
Description	Pseudogenes are copies of already existing genes, and while their sequences resemble the original parental gene, they contain mutations which generally render them non-functional. However, recent advances in genome analysis suggest that pseudogenes can in fact be translated and have specific functions. Ubiquitin (Ub) is an abundant protein transcribed from four different genes, increasing the potential for the generation of several pseudogenes. Through human genome analysis datamining, we gathered information regarding Ub and Ub like (Ubl) pseudogenes in order to identify potentially functional variants. Through experimental validation, we found a candidate gene RPS27AP5 which not only expresses a Ub (UbP5) but a ribosomal protein (S27aP5) as well. The precursor fusion protein is matured through cleavage and both proteins behave as their respective counterparts following translation. S27aP5 is able to integrate into translating ribosomes and its expression increases the 80S, monosomal, ribosomal fraction. The existence of a functional S27a pseudogene supports the idea that ribosomes are flexible complexes and that by exchanging certain subunits; they can perform specific translational functions giving rise to specialised ribosomes.
HostingRepository	PRIDE
AnnounceDate	2026-02-04
AnnouncementXML	Submission_2026-02-03_16:45:50.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Levesque
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-05 12:23:27	ID requested
⏵ 1	2026-02-03 16:45:51	announced

10.1139/bcb-2025-0228;

Meller A, Raisch J, L, é, vesque D, Fafard-Couture E, Scott M, Roucou X, Boisvert FM, The pseudogene RPS27AP5 expresses ubiquitin and ribosomal protein variants with potential roles in ribosome function. Biochem Cell Biol, 103():1-18(2025) [pubmed]

submitter keyword: pseudogene, ribosome, ubiquitin, translation

François-Michel Boisvert
contact affiliation	Department of Immunology and Cell biology, Université de Sherbrooke, 3201, Jean-Mignault, Sherbrooke, Québec, J1E 4K8, Canada
contact email	francois.michel.boisvert@usherbrooke.ca
lab head
Dominique Levesque
contact affiliation	University of Sherbrooke, FM Boisvert lab
contact email	dominique.levesque@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047544
     1. Label: PRIDE project
     2. Name: Ribosomal protein S27A pseudogene 5 is involved in monosome related RNA translation