PXD047514
PXD047514 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometric profiling of lysine malonylation in CRISPRi 562 cells lines to investigate malonylation activity of lysine acetyltransferases |
Description | Lysine malonylation is a protein posttranslational modification with potentially broad biological impact. We present a protocol to generate stable gene knockdown K562 cell lines through lentiviral infection of a CRISPRi system followed by lysine malonylation measurement using mass spectrometry (MS). We detail gRNA vector cloning, lentiviral infection, cell line purification, protein digestion, malonyllysine enrichment, desalting, and MS acquisition and analysis. This protocol can be applied for CRISPRa cell line generation and lysine malonylation profiling in tissues, with slight changes. |
HostingRepository | MassIVE |
AnnounceDate | 2024-05-09 |
AnnouncementXML | Submission_2024-05-09_08:44:15.853.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joanna Bons |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | N6-malonyl-L-lysine |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-12-04 15:09:51 | ID requested | |
⏵ 1 | 2024-05-09 08:44:16 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Malonylation, Data-independent acquisition (DIA), Post-translational modifications, Lysine acetyltransferases, Stable gene knockdown K562 cell lines |
Contact List
Birgit Schilling | |
---|---|
contact affiliation | Buck Institute |
contact email | bschilling@buckinstitute.org |
lab head | |
Joanna Bons | |
contact affiliation | Buck Institute for Research on Aging |
contact email | jbons@buckinstitute.org |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093564/ |