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PXD047514

PXD047514 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass spectrometric profiling of lysine malonylation in CRISPRi 562 cells lines to investigate malonylation activity of lysine acetyltransferases
DescriptionLysine malonylation is a protein posttranslational modification with potentially broad biological impact. We present a protocol to generate stable gene knockdown K562 cell lines through lentiviral infection of a CRISPRi system followed by lysine malonylation measurement using mass spectrometry (MS). We detail gRNA vector cloning, lentiviral infection, cell line purification, protein digestion, malonyllysine enrichment, desalting, and MS acquisition and analysis. This protocol can be applied for CRISPRa cell line generation and lysine malonylation profiling in tissues, with slight changes.
HostingRepositoryMassIVE
AnnounceDate2024-05-09
AnnouncementXMLSubmission_2024-05-09_08:44:15.853.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoanna Bons
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListN6-malonyl-L-lysine
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-04 15:09:51ID requested
12024-05-09 08:44:16announced
Publication List
no publication
Keyword List
submitter keyword: Malonylation, Data-independent acquisition (DIA), Post-translational modifications, Lysine acetyltransferases, Stable gene knockdown K562 cell lines
Contact List
Birgit Schilling
contact affiliationBuck Institute
contact emailbschilling@buckinstitute.org
lab head
Joanna Bons
contact affiliationBuck Institute for Research on Aging
contact emailjbons@buckinstitute.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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