PXD047498

PXD047498 is an original dataset announced via ProteomeXchange.

Title	Suppression of the E. coli rnpA49 conditionally lethal phenotype via different compensatory mutations
Description	RNase P is an essential enzyme found across all domains of life that is responsible for the 5’-end maturation of precursor tRNA transcripts. Since its discovery in the 1970s, numerous studies have sought to elucidate the mechanisms and biochemistry governing RNase P function. However, much remains unknown about the regulation of RNase P expression, the turnover and degradation of the enzyme, and the mechanisms underlying the phenotypes and complementation of specific RNase P mutations. In Escherichia coli, the temperature-sensitive rnpA49 mutation in the protein subunit of RNase P has arguably been one of the most well-studied and commonly used mutations for examining the enzyme’s activity in vivo. Here we report for the first time naturally-occurring temperature-resistant suppressor mutations of E. coli strains carrying the rnpA49 allele. We find that rnpA49 strains can partially compensate the temperature-sensitive defect via gene amplifications of either RNase P subunit (rnpA49 or rnpB) or by the acquisition of loss-of-function mutations in Lon protease or RNase R. Our results agree with previous plasmid overexpression and gene deletion complementation studies and importantly suggest the involvement of Lon protease in the degradation and/or regulatory pathway(s) of the mutant protein subunit of RNase P. This work offers novel insight into the behavior and complementation of the rnpA49 allele in vivo and provides direction for follow-up studies regarding RNase P regulation and turnover.
HostingRepository	PRIDE
AnnounceDate	2024-05-15
AnnouncementXML	Submission_2024-05-15_04:20:18.915.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evelin Berger
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-04 01:54:57	ID requested
⏵ 1	2024-05-15 04:20:19	announced

Babina AM, Kirsebom LA, Andersson DI, conditionally lethal phenotype by different compensatory mutations. RNA, 30(8):977-991(2024) [pubmed]

10.1261/rna.079909.123;

submitter keyword: RNase P, RNase R, Lon, Suppressor mutation, Gene amplification, Temperature-sensitivity

Carina Sihlbom
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Sweden
contact email	carina.sihlbom@gu.se
lab head
Evelin Berger
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	evelin.berger@gu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047498
     1. Label: PRIDE project
     2. Name: Suppression of the E. coli rnpA49 conditionally lethal phenotype via different compensatory mutations