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PXD047497

PXD047497 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of proteins in ribosomal fractions isolated from sporulating Bacillus subtilis
DescriptionThe aim of the project was to investigate polysome profiles and analyse ribosomes of sporulating Bacillus subtilis strains, WT and a triple knock-out (3KO) strain lacking three paralogs of ribosomal proteins, by mass spectrometry, depending on the sample preparation conditions. B. subtilis, a gram positive spore forming bacterium, was induced to sporulate and harvested three hours post sporulation induction, approximately soon after asymmetric septation, respectively. Three conditions were analysed: 1) proteins were cross-linked by addition of DSP to the culture just before harvesting, and the cells were lysed by membrane solubilisation with digitonin, 2) cell lysis was performed by membrane solubilisation with digitonin with no prior cross-linking, 3) cell lysis was performed by grinding with pestle and mortar with addition of aluminium oxide. To provide additional comparisons, analogous experiments were performed for the cultures collected in logarithmic growth phase and two hours post sporulation induction, before asymmetric septation (lysis by membrane solubilisation with digitonin and grinding respectively). For the 3KO strain, cultures were harvested 3hours post sporulation induction and lysates were prepared by grinding.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:50:38.573.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRemigiusz Serwa
SpeciesList scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-04 01:37:37ID requested
12024-07-17 01:03:20announced
22024-10-22 06:50:38announced2024-10-22: Updated project metadata.
Publication List
10.1101/2023.12.06.569898;
Keyword List
submitter keyword: Bacillus subtilis, ribosome, sporulation
Contact List
Agata Starosta
contact affiliationInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, Warsaw 02-106, Poland
contact emailagata.starosta@ibb.waw.pl
lab head
Remigiusz Serwa
contact affiliationThe International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact emailr.serwa@imol.edu.pl
dataset submitter
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Dataset FTP location
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