PXD047487
PXD047487 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A novel capsid-XL32-derived adeno-associated virus serotype prompts retinal tropism and ameliorates choroidal neovascularization |
Description | Gene therapy has been adapted, from the laboratory to the clinic, to treat retinopathies. In contrast to subretinal route, intravitreal delivery of AAV vectors displays the advantage of bypassing surgical injuries, but the viral particles are more prone to be nullified by the host neutralizing factors. To minimize such suppression of therapeutic effect, especially in terms of AAV2 and its derivatives, we introduced three serine-to-glycine mutations, based on the phosphorylation sites identified by mass spectrum analysis, to the XL32 capsid to generate a novel serotype named AAVYC5. Via intravitreal administration, AAVYC5 was transduced more effectively into multiple retinal layers compared with AAV2 and XL32. AAVYC5 also enabled successful delivery of anti-angiogenic molecules to rescue laser-induced choroidal neovascularization and astrogliosis in mice and non-human primates. Furthermore, we detected fewer neutralizing antibodies and binding IgG in human sera against AAVYC5 than those specific for AAV2 and XL32. Our results thus implicate this capsid-optimized AAVYC5 as a promising vector suitable for a wide population, particularly those with undesirable AAV2 seroreactivity. |
HostingRepository | iProX |
AnnounceDate | 2023-12-04 |
AnnouncementXML | Submission_2024-05-15_01:17:25.908.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yuqiu Wang |
SpeciesList | scientific name: Adeno-Associated Virus PREDICT_AaV-6; NCBI TaxID: 1717199; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-12-03 19:47:57 | ID requested | |
1 | 2023-12-03 19:54:48 | announced | |
⏵ 2 | 2024-05-15 01:17:26 | announced | 2024-05-15: Update information. |
Publication List
Luo LL, Xu J, Wang BQ, Chen C, Chen X, Hu QM, Wang YQ, Zhang WY, Jiang WX, Li XT, Zhou H, Xiao X, Zhao K, Lin S, A novel capsid-XL32-derived adeno-associated virus serotype prompts retinal tropism and ameliorates choroidal neovascularization. Biomaterials, 304():122403(2024) [pubmed] |
Keyword List
submitter keyword: AAVXL32 |
Contact List
Hu Zhou | |
---|---|
contact affiliation | Shanghai Institute of Materia Medica |
contact email | zhouhu@simm.ac.cn |
lab head | |
Yuqiu Wang | |
contact affiliation | Shanghai Institute of Materia Medica |
contact email | 995752924@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |