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PXD047487

PXD047487 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA novel capsid-XL32-derived adeno-associated virus serotype prompts retinal tropism and ameliorates choroidal neovascularization
DescriptionGene therapy has been adapted, from the laboratory to the clinic, to treat retinopathies. In contrast to subretinal route, intravitreal delivery of AAV vectors displays the advantage of bypassing surgical injuries, but the viral particles are more prone to be nullified by the host neutralizing factors. To minimize such suppression of therapeutic effect, especially in terms of AAV2 and its derivatives, we introduced three serine-to-glycine mutations, based on the phosphorylation sites identified by mass spectrum analysis, to the XL32 capsid to generate a novel serotype named AAVYC5. Via intravitreal administration, AAVYC5 was transduced more effectively into multiple retinal layers compared with AAV2 and XL32. AAVYC5 also enabled successful delivery of anti-angiogenic molecules to rescue laser-induced choroidal neovascularization and astrogliosis in mice and non-human primates. Furthermore, we detected fewer neutralizing antibodies and binding IgG in human sera against AAVYC5 than those specific for AAV2 and XL32. Our results thus implicate this capsid-optimized AAVYC5 as a promising vector suitable for a wide population, particularly those with undesirable AAV2 seroreactivity.
HostingRepositoryiProX
AnnounceDate2023-12-04
AnnouncementXMLSubmission_2024-05-15_01:17:25.908.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuqiu Wang
SpeciesList scientific name: Adeno-Associated Virus PREDICT_AaV-6; NCBI TaxID: 1717199;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-03 19:47:57ID requested
12023-12-03 19:54:48announced
22024-05-15 01:17:26announced2024-05-15: Update information.
Publication List
Luo LL, Xu J, Wang BQ, Chen C, Chen X, Hu QM, Wang YQ, Zhang WY, Jiang WX, Li XT, Zhou H, Xiao X, Zhao K, Lin S, A novel capsid-XL32-derived adeno-associated virus serotype prompts retinal tropism and ameliorates choroidal neovascularization. Biomaterials, 304():122403(2024) [pubmed]
Keyword List
submitter keyword: AAVXL32
Contact List
Hu Zhou
contact affiliationShanghai Institute of Materia Medica
contact emailzhouhu@simm.ac.cn
lab head
Yuqiu Wang
contact affiliationShanghai Institute of Materia Medica
contact email995752924@qq.com
dataset submitter
Full Dataset Link List
iProX dataset URI