PXD047478

PXD047478 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAc proteome analysis by Bioorthogonal Ligation-Tryptic Cleavage Strategy
Description	Protein O-GlcNAcylation, an extensive post-translational modification (PTM), has been proposed as a nutrient and stress sensor that regulates multiple essential cellular processes. Due to the low stoichiometry of O-GlcNAcylation and the high complexity of biological samples, comprehensive analysis of O-GlcNAcylation is an extraordinary challenge. In this work, we established a novel, simple, site-specific O-GlcNAc identification strategy by integrating bioorthogonal ligation and tryptic-cleavage，and applied it for O-GlcNAc proteomics analysis and applications. In this strategy, the capture step was based on click chemistry reaction between solid material and peptides, and the release step was completed by efficient tryptic cleavage. Benefiting from the simplified workflow and a tryptic-cleavable tag, this strategy would be a versatile tool for the proteomic analysis of O-GlcNAcylation to explore O-GlcNAcylation functions involving in cellular physiology and pathology disease.
HostingRepository	iProX
AnnounceDate	2023-12-01
AnnouncementXML	Submission_2023-12-03_19:16:19.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingying Xiong
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-03 19:15:36	ID requested
⏵ 1	2023-12-03 19:16:20	announced

Dataset with its publication pending

submitter keyword: O-GlcNAcylation, Glycoproteomics

Haojie Lu
contact affiliation	Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Yingying Xiong
contact affiliation	Fudan University
contact email	20110220039@fudan.edu.cn
dataset submitter

iProX dataset URI