PXD047466

PXD047466 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics reveals oxygen-induced adaptations in Caldalkalibacillus thermarum TA2.A1 chemostat cultures
Description	Caldalkalibacillus thermarum possesses a highly branched respiratory chain. Branched electron transport chains (ETC) in model organisms such as Escherichia coli primarily facilitate growth at a wide range of dissolved oxygen levels. The aim of this study was to investigate whether a polyextremophile such as C. thermarum is regulated in a similar fashion. With this in mind, the organism was cultivated in chemostat bioreactors with a range of oxygen levels in the inlet gas (0.25% O2 – 4.2% O2). Proteomic analysis unexpectedly showed both the type I and the type II NADH dehydrogenases present in all conditions. Moreover, also two different terminal oxidases were present. The cytochrome c:oxygen aa3 complex abundance was highest at 4.2% O2, while the cytochrome c:oxygen ba3 complex exhibited higher abundance at the lowest O2 concentration 0.25% O2. The abundance of the cytochrome c:oxygen ba3 complex started to decline below 0.42% O2. Often this would result in emergence of the cytochrome c:oxygen bb3 complex or the menaquinol:oxygen bd complex, the other two terminal oxidases of C. thermarum; but neither was detected. Besides changes in the respiratory chain, the sodium-proton antiporter complex Mrp was downregulated under the lower oxygen levels. Normally, in alkaliphiles, this enzyme is considered crucial for sodium homeostasis. We propose that the existence of a sodium:acetate exporter decreases the requirement for Mrp under strong oxygen limitation, introducing a novel perspective to the field of alkaliphiles. contact: s.i.dejong@tudelft.nl or d.g.g.mcmillan@tudelft.nl
HostingRepository	PRIDE
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-17_22:28:55.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Pabst
SpeciesList	scientific name: Caldalkalibacillus thermarum TA2.A1; NCBI TaxID: 986075;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-02 17:37:29	ID requested
⏵ 1	2025-01-17 22:28:56	announced

Dataset with its publication pending

submitter keyword: shotgun proteomics,Caldalkalibacillus thermarum, oxygen adaptation

Martin Pabst
contact affiliation	Department of Biotechnology, TU Delft, The Netherlands
contact email	m.pabst@tudelft.nl
lab head
Martin Pabst
contact affiliation	TUD
contact email	m.pabst@tudelft.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047466
     1. Label: PRIDE project
     2. Name: Quantitative proteomics reveals oxygen-induced adaptations in Caldalkalibacillus thermarum TA2.A1 chemostat cultures