PXD047458

PXD047458 is an original dataset announced via ProteomeXchange.

Title	RNA sequencing and proteomic profiling reveal alterations by MPTP in chronic stomach mucosal injury in tree shrew Chinese (Tupaia belangeri chinensis)
Description	1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a neurotoxin that can cause gastrointestinal ulcers by affecting dopamine levels. Therefore, MPTP has been considered a toxic substance that causes gastric ulcer disease in experimental animals. In this study, tree shrews were used as the animal model of gastric mucosa injury, and MPTP was intraperitoneally injected for 13 weeks, while tree shrews were not injected as the control group. Under the light microscope, local congestion or diffuse bleeding points of gastric mucosa and multiple redness and swelling bleeding symptoms on the inner wall were observed in the treatment group, as well as immune cell infiltration was found in HE staining, but no such phenomenon was observed in the control group. In order to explore the molecular basis of changes in MPTP induced gastric mucosa injury, the transcriptome and proteome data of gastric mucosa were analyzed. We observed significant differences in mRNA and protein expression levels under the influence of MPTP. The changes in mRNA and proteins are related to increased immune infiltration, cellular processes and angiogenesis. More differentially expressed genes play a role in immune function, especially the candidate genes RPL4 and ANXA1 with significant signal and core role. There are also differentially expressed genes that play a role in mucosal injury and shedding, especially candidate genes GAST and DDC with certain signaling and corresponding functions. Understanding the factors and molecular basis that affect the expression of related genes is crucial for coping with Emotionality gastric mucosa injury disease and developing new treatment methods to establish the ability to resist disease.
HostingRepository	PRIDE
AnnounceDate	2024-02-12
AnnouncementXML	Submission_2024-02-12_09:03:45.127.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tang Dong hong
SpeciesList	scientific name: Tupaia belangeri; NCBI TaxID: 37347;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-02 02:13:14	ID requested
⏵ 1	2024-02-12 09:03:46	announced

10.1038/s41598-023-50820-y;

Wang CY, Ye YS, Long WH, Li ZL, Zheng H, Lin XR, Zhou W, Tang DH, RNA sequencing and proteomic profiling reveal alterations by MPTP in chronic stomach mucosal injury in tree shrew Chinese (Tupaia belangeri chinensis). Sci Rep, 14(1):74(2024) [pubmed]

submitter keyword: RNA sequencing ,MPTP, chronic stomach mucosal injury, proteomic profiling, tree shrew

Dong-hong Tang
contact affiliation	Medical Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences/Peking Union Medical College, Kunming , China
contact email	783849021@qq.com
lab head
Tang Dong hong
contact affiliation	Institute of Medical Biology, Chinese Academy of Medical Sciences/Peking Union Medical College
contact email	783849021@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047458
     1. Label: PRIDE project
     2. Name: RNA sequencing and proteomic profiling reveal alterations by MPTP in chronic stomach mucosal injury in tree shrew Chinese (Tupaia belangeri chinensis)