PXD047447

PXD047447 is an original dataset announced via ProteomeXchange.

Title	Membrane Vesicles in Acidithiobacillia class extreme acidophiles: influence on collective behaviours of 'Fervidacidithiobacillus caldus'
Description	Membrane Vesicles (MVs) are envelope derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied in pathogenic microorganisms, the roles, relevance and biotechnological potential of MVs from environmental bacteria are less well established. Acidithiobacillaceae family bacteria are active players in the sulfur and iron biogeochemical cycles in extreme acidic environments and drivers of the leaching of mineral ores contributing to acid rock/mine drainage (ARD/AMD) and industrial bioleaching. One key aspect to such rol is the ability of these bacteria to tightly interact with the mineral surfaces and extract electrons and nutrients to support their chemolithotrophic metabolism. Despite recent advances in the characterization of acidithiobacilli biofilms and extracellular matrix (ECM) components, our understanding of its architectural and mechanistic aspects remains scant. In this work, we show that vesiculation is a common phenomenon in distant members of the Acidithiobacillaceae family, and further explore the role of MVs in multicellular colonization behaviours using `Fervidacidithiobacillus caldus´ as bacterial model. Production of MVs in `F. caldus´ occurred in both planktonic cultures and biofilms formed on sulfur surfaces, where MVs appeared individually or in chains resembling Tube-Shaped Membranous Structures (TSMSs) important for microbial communication. Liquid chromatography-mass spectrometry data and bioinformatic analysis of the MVs-associated proteome revealed that F. caldus MVs were enriched in proteins involved in cell-cell and cell-surface processes, and largely typified the MVs as outer MVs (OMVs). Finally, microbiological assays showed that amendment of `F. caldus´ MVs to cells and/or biofilms affects collective colonizing behaviours relevant to the ecophysiology and applications of these acidophiles providing grounds for their exploitation in mining biotechnologies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:55.497.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillermo Nourdin
SpeciesList	scientific name: Acidithiobacillaceae; NCBI TaxID: 225058;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-01 07:39:24	ID requested
1	2023-12-29 21:02:53	announced
⏵ 2	2024-10-22 06:18:55	announced	2024-10-22: Updated project metadata.

10.3389/FMICB.2023.1331363;

submitter keyword: Outer Membrane Vesicles (OMVs), Biofilm, Tube-Shaped Membranous Structures (TSMSs), Acidithiobacillus, Adhesins, Swarming, Surface Colonization, Sulfur Oxidation

Matías Castro
contact affiliation	Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile. Instituto Milenio de Oceanografía (IMO), Universidad de Concepción, Concepción, Chile.
contact email	matias.castro@imo-chile.cl
lab head
Guillermo Nourdin
contact affiliation	MELISA Institute
contact email	gnourdin@melisainstitute.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047447

PRIDE project URI

• PRIDE
  1. PXD047447
     1. Label: PRIDE project
     2. Name: Membrane Vesicles in Acidithiobacillia class extreme acidophiles: influence on collective behaviours of 'Fervidacidithiobacillus caldus'