PXD047445

PXD047445 is an original dataset announced via ProteomeXchange.

Title	Visfatin impact on the proteome of porcine luteal cells during implantation
Description	Visfatin (VIS) is a hormone belonging to the adipokines’ group secreted mainly by the adipose tissue. VIS plays a crucial role in the control of energy homeostasis, inflammation, cell differentiation, and angiogenesis. VIS expression was confirmed in the hypothalamic-pituitary-gonadal (HPG) axis structures, as well as in the uterus, placenta, and conceptuses. We assumed that VIS may affect the abundance of proteins involved in the regulation of key processes occurring in the corpus luteum (CL) during the implantation process. In the present study, we performed the high-throughput proteomic analysis (liquid chromatography with tandem mass spectrometry, LC-MS/MS) to examine the influence of VIS (100 ng/mL) on differentially regulated proteins (DRPs) in the porcine luteal cells (LCs) on days 15-16 of pregnancy (implantation period). We have identified 511 DRPs, 276 of them were up-regulated and 235 down-regulated in the presence of VIS. Revealed DRPs were assigned to 162 gene ontology terms. Western blot analysis of five chosen DRPs, ADAM metallopeptidase with thrombospondin type 1 motif 1 (ADAMTS1), lanosterol 14-α demethylase (CYP51A1), inhibin subunit beta A (INHBA), notch receptor 3 (NOTCH3), and prostaglandin E synthase 2 (mPGES2) confirmed the veracity and accuracy of LC-MS/MS method. We indicated that VIS modulates the expression of proteins connected with the regulation of lipogenesis and cholesterologenesis, and, in consequence, may be involved in the synthesis of steroid hormones, as well as prostaglandins’ metabolism. VIS also changes the expression of proteins involved in the insulin signalling pathway. Moreover, we revealed that VIS affects the abundance of protein associated with ovarian cell proliferation, differentiation, and apoptosis, as well as CL new vessel formation and tissue remodelling. Our results seem to confirm the hypothesis concerning the important role of VIS in the regulation of ovarian functions during the peri-implantation period.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:48:35.065.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047445
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-01 06:46:11	ID requested
1	2024-07-03 02:15:07	announced
⏵ 2	2024-10-22 06:48:39	announced	2024-10-22: Updated project metadata.

10.6019/PXD047445;

Kopij G, Kiezun M, Gudelska M, Dobrzyn K, Zarzecka B, Rytelewska E, Zaobidna E, Swiderska B, Malinowska A, Rak A, Kaminski T, Smolinska N, Visfatin impact on the proteome of porcine luteal cells during implantation. Sci Rep, 14(1):14625(2024) [pubmed]

10.1038/s41598-024-65577-1;

submitter keyword: implantation, early pregnancy, LC-MS, luteal cells, corpora lutea, proteome, pig,visfatin

Dr Nina Smolińska
contact affiliation	Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury
contact email	nina.smolinska@uwm.edu.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD047445

PRIDE project URI

• PRIDE
  1. PXD047445
     1. Label: PRIDE project
     2. Name: Visfatin impact on the proteome of porcine luteal cells during implantation