⮝ Full datasets listing

PXD047440

PXD047440 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleControl of a type III-Dv CRISPR-Cas system by the transcription factor RpaB and interaction of its transcribed leader with the DEAD-box RNA helicase CrhR
DescriptionCRISPR-Cas systems in bacteria and archaea provide powerful defense against phages and other foreign genetic elements. The principles of CRISPR-Cas activity are well understood, but less is known about how their expression is regulated. The cyanobacterium Synechocystis sp. PCC 6803 encodes three separate CRISPR-Cas systems. The expression of one of these, a type III-Dv system, responds to changes in environmental conditions such as nitrogen starvation or changing light intensities. Here, we found that the promoter of the respective six-gene cas operon is controlled by the light- and redox-responsive transcription factor RpaB. RpaB binds to an HLR1 motif 53 to 70 nt upstream of the transcriptional start site, leading to the activation of transcription at low light intensities. However, the strong promoter driving transcription of the cognate repeat-spacer array is not under RpaB control. Instead, we found the 125 nt transcribed leader to be bound by the redox-sensitive RNA helicase CrhR, and crosslinking coupled to mass spectrometry analysis revealed six residues specifically interacting with it. From these, L103, F104, H225, and C371 were predicted on the surface of a dimeric CrhR model. These results show that the expression of the CRISPR-Cas system is linked to the redox status of the photosynthetic cyanobacterial cell and that the elements involved are unique in that they act at two different levels. While RpaB affects transcription, CrhR engages with the transcribed leader at the post-transcriptional level. These results highlight the complex nature of the interrelationships between a CRISPR-Cas system and its host cell.
HostingRepositoryPRIDE
AnnounceDate2024-07-15
AnnouncementXMLSubmission_2024-07-15_08:58:47.409.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlexandr Dybkov
SpeciesList scientific name: Synechocystis sp. (strain PCC 6803 / Kazusa); NCBI TaxID: 1111708;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-01 04:20:35ID requested
12024-07-15 08:58:47announced
Publication List
10.1016/J.CELREP.2024.114485;
Keyword List
submitter keyword: cyanobacteria, CrhR DEAD-box RNA helicase, CRISPR leader, transcription factor RpaB,CRISPR-Cas systems, regulation of CRISPR-Cas gene expression
RNA structure
Contact List
Olexandr Dybkov
contact affiliationBioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Germany
contact emailodybkov@gwdg.de
lab head
Olexandr Dybkov
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailodybkov@gwdg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD047440
PRIDE project URI
Repository Record List
[ + ]