PXD047430
PXD047430 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Affinity purification of TRMT6/61A protein complexes |
Description | In order to identify the molecular mechanism leading to the increase of TRMT6/61A complex in aged HSCs, we purified proteins interacting with TRMT6 and TRMT61A via affinity purification. HEK293T cells stably expressing SFB-tagged TRMT6 or SFB-TRMT61A were lysed in NETN buffer (20 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1 mM EDTA, and 0.5% Non- idet P-40, containing protease and phosphatase inhibitors cocktail) on ice for 30 min. Cell debris were removed by centrifugation and the supernatant were collected and incubated with 50 μL S-protein beads for 4 hr at 4 ℃. Beads were washed with 1 mL NTEN buffer for three times, the immunocomplexes were boiled in 20 μL 2 x SDS loading buffer. Samples were resolved on 10% SDS-PAGE and analyzed by mass spectrometry. |
HostingRepository | iProX |
AnnounceDate | 2023-11-30 |
AnnouncementXML | Submission_2024-01-18_18:37:30.167.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hanqing He |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-30 21:31:05 | ID requested | |
⏵ 1 | 2024-01-18 18:37:30 | announced |
Publication List
He H, Wang Y, Zhang X, Li X, Liu C, Yan D, Deng H, Sun W, Yi C, Wang J, Age-related noncanonical TRMT6-TRMT61A signaling impairs hematopoietic stem cells. Nat Aging, 4(2):213-230(2024) [pubmed] |
Keyword List
submitter keyword: TRMT6/61A complex |
Contact List
Jianwei Wang | |
---|---|
contact affiliation | Tsinghua University |
contact email | wangjianwei@ihcams.ac.cn |
lab head | |
Hanqing He | |
contact affiliation | Tsinghua University |
contact email | hhq18@tsinghua.org.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |