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PXD047430

PXD047430 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAffinity purification of TRMT6/61A protein complexes
DescriptionIn order to identify the molecular mechanism leading to the increase of TRMT6/61A complex in aged HSCs, we purified proteins interacting with TRMT6 and TRMT61A via affinity purification. HEK293T cells stably expressing SFB-tagged TRMT6 or SFB-TRMT61A were lysed in NETN buffer (20 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1 mM EDTA, and 0.5% Non- idet P-40, containing protease and phosphatase inhibitors cocktail) on ice for 30 min. Cell debris were removed by centrifugation and the supernatant were collected and incubated with 50 μL S-protein beads for 4 hr at 4 ℃. Beads were washed with 1 mL NTEN buffer for three times, the immunocomplexes were boiled in 20 μL 2 x SDS loading buffer. Samples were resolved on 10% SDS-PAGE and analyzed by mass spectrometry.
HostingRepositoryiProX
AnnounceDate2023-11-30
AnnouncementXMLSubmission_2024-01-18_18:37:30.167.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHanqing He
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-30 21:31:05ID requested
12024-01-18 18:37:30announced
Publication List
He H, Wang Y, Zhang X, Li X, Liu C, Yan D, Deng H, Sun W, Yi C, Wang J, Age-related noncanonical TRMT6-TRMT61A signaling impairs hematopoietic stem cells. Nat Aging, 4(2):213-230(2024) [pubmed]
Keyword List
submitter keyword: TRMT6/61A complex
Contact List
Jianwei Wang
contact affiliationTsinghua University
contact emailwangjianwei@ihcams.ac.cn
lab head
Hanqing He
contact affiliationTsinghua University
contact emailhhq18@tsinghua.org.cn
dataset submitter
Full Dataset Link List
iProX dataset URI