PXD047430

PXD047430 is an original dataset announced via ProteomeXchange.

Title	Affinity purification of TRMT6/61A protein complexes
Description	In order to identify the molecular mechanism leading to the increase of TRMT6/61A complex in aged HSCs, we purified proteins interacting with TRMT6 and TRMT61A via affinity purification. HEK293T cells stably expressing SFB-tagged TRMT6 or SFB-TRMT61A were lysed in NETN buffer (20 mM Tris-HCl [pH 8.0], 100 mM NaCl, 1 mM EDTA, and 0.5% Non- idet P-40, containing protease and phosphatase inhibitors cocktail) on ice for 30 min. Cell debris were removed by centrifugation and the supernatant were collected and incubated with 50 μL S-protein beads for 4 hr at 4 ℃. Beads were washed with 1 mL NTEN buffer for three times, the immunocomplexes were boiled in 20 μL 2 x SDS loading buffer. Samples were resolved on 10% SDS-PAGE and analyzed by mass spectrometry.
HostingRepository	iProX
AnnounceDate	2023-11-30
AnnouncementXML	Submission_2024-01-18_18:37:30.167.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hanqing He
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-30 21:31:05	ID requested
⏵ 1	2024-01-18 18:37:30	announced

submitter keyword: TRMT6/61A complex

Jianwei Wang
contact affiliation	Tsinghua University
contact email	wangjianwei@ihcams.ac.cn
lab head
Hanqing He
contact affiliation	Tsinghua University
contact email	hhq18@tsinghua.org.cn
dataset submitter

iProX dataset URI