PXD047416

PXD047416 is an original dataset announced via ProteomeXchange.

Title	Mapping myddosome components in human and murine macrophages.
Description	Toll-like Receptors (TLRs) are key mediators of immune responses to infection. Upon microbial detection, these receptors activate inflammatory signal transduction pathways that involve IκB kinases, mitogen activated protein kinases, ubiquitin ligases and other adaptor proteins. Current models suggest these signaling proteins operate within functionally distinct multiprotein complexes, which are all activated by a receptor-proximal complex known as the myddosome. The mechanisms that connect the effector protein complexes in the TLR pathways are undefined. To define TLR pathway activities, we engineered macrophages to enable proteomic analysis of the endogenous myddosome constituent MyD88 and its interacting proteins. Proteomics demonstrated that myddosomes contain proteins that act at all stages and regulate all effector responses of the TLR signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2024-07-04
AnnouncementXML	Submission_2024-07-04_10:59:15.349.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Fisch
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-30 13:45:08	ID requested
⏵ 1	2024-07-04 10:59:15	announced

10.1038/S41586-024-07614-7;

submitter keyword: LPS, myddosome, APEX, TMT, human, MyD88, proximity-biotinylation, Toll-like receptor, mouse, PAMP,Macrophage, P3C

Jonathan C Kagan
contact affiliation	Division of Gastroenterology, Boston Children's Hospital and Harvard Medical School, 300 Longwood Ave., Boston, MA 02115, USA
contact email	jonathan.kagan@childrens.harvard.edu
lab head
Daniel Fisch
contact affiliation	Boston Children's Hospital
contact email	daniel.fisch@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047416
     1. Label: PRIDE project
     2. Name: Mapping myddosome components in human and murine macrophages.