PXD047404

PXD047404 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human 293T cells lysosomal osmotic stress response
Description	The goal of this project is to identify changes of the lysosomal surface proteome upon the induction of lysosomal vacuolation. 293T cells stably expressing LAMP1-GFP-TurboID or Lyso-TurboID were treated with a small molecule apilimod or DMSO to trigger lysosomal vacuolation, after which the lysosomal surface proteins were labeled with biotin by TurboID. The biotinylated proteins by Lyso-TurboID were purified by streptavidin beads for mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_04:40:59.757.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047404
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jay Xiaojun Tan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-30 07:55:02	ID requested
⏵ 1	2025-08-21 04:41:00	announced

Publication List

10.6019/PXD047404;

10.6019/PXD047404;

Keyword List

submitter keyword: Lysosomal vacuolation, streptavidin purification, lysosomal surface proteome, biotinylation

submitter keyword: Lysosomal vacuolation, streptavidin purification, lysosomal surface proteome, biotinylation

Contact List

Jay Xiaojun Tan, PhD
contact affiliation	Aging Institute Department of Cell Biology University of Pittsburgh School of Medicine/UPMC Bridgeside Point I Room 457 100 Technology Dr. Pittsburgh, PA 15219
contact email	Jay.Tan@pitt.edu
lab head
Jay Xiaojun Tan
contact affiliation	University of Pittsburgh
contact email	jay.tan@pitt.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD047404
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD047404

PRIDE project URI

Repository Record List

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