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PXD047397

PXD047397 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSILAC experiment of HEK 293 cells upon RPS26 silencing
DescriptionIn recent years, there is emerging pool of evidence suggesting that ribosomal composition plays a role in selection of transcripts that are actively translated at given time and tissue. Some ribosomal subunits are not the core components of 40S ribosome and alternatively can be assembled or disassembled from 40S subunit. For example, a 40S ribosomal protein - RPS26 is one of such alternative ribosomal subunits. In ordered to investigate what proteins are under the RPS26-depended translation, we performed stable isotope labeling using amino acids in cell culture (SILAC) experiment 48 hours post siRNA-based RPS26 silencing in HEK293 cells and revealed the pool of proteins that are up or downregulated upon RPS26 depletion.
HostingRepositoryPRIDE
AnnounceDate2025-05-26
AnnouncementXMLSubmission_2025-05-25_16:12:55.451.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnna Baud
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-30 04:08:20ID requested
12025-05-25 16:12:56announced
Publication List
10.7554/elife.98631;
Tutak K, Broniarek I, Zielezinski A, Niewiadomska D, Skrzypczak T, Baud A, Sobczak K, Insufficiency of 40S ribosomal proteins, RPS26 and RPS25, negatively affects biosynthesis of polyglycine-containing proteins in fragile-X associated conditions. Elife, 13():(2025) [pubmed]
Keyword List
submitter keyword: RPS26, human, SILAC
Contact List
Krzysztof Sobczak
contact affiliationDepartment of Gene Expression Institute of Molecular Biology and Biotechnology Faculty of Biology Adam Mickiewicz University Uniwersytetu Poznanskiego 6 61-614 Poznan
contact emailksobczak@amu.edu.pl
lab head
Anna Baud
contact affiliationAdam Mickiewicz University in Poznan
contact emailannbau1@amu.edu.pl
dataset submitter
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Dataset FTP location
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