PXD047397 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SILAC experiment of HEK 293 cells upon RPS26 silencing |
Description | In recent years, there is emerging pool of evidence suggesting that ribosomal composition plays a role in selection of transcripts that are actively translated at given time and tissue. Some ribosomal subunits are not the core components of 40S ribosome and alternatively can be assembled or disassembled from 40S subunit. For example, a 40S ribosomal protein - RPS26 is one of such alternative ribosomal subunits. In ordered to investigate what proteins are under the RPS26-depended translation, we performed stable isotope labeling using amino acids in cell culture (SILAC) experiment 48 hours post siRNA-based RPS26 silencing in HEK293 cells and revealed the pool of proteins that are up or downregulated upon RPS26 depletion. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-26 |
AnnouncementXML | Submission_2025-05-25_16:12:55.451.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anna Baud |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C labeled residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-30 04:08:20 | ID requested | |
⏵ 1 | 2025-05-25 16:12:56 | announced | |
Publication List
10.7554/elife.98631; |
Tutak K, Broniarek I, Zielezinski A, Niewiadomska D, Skrzypczak T, Baud A, Sobczak K, Insufficiency of 40S ribosomal proteins, RPS26 and RPS25, negatively affects biosynthesis of polyglycine-containing proteins in fragile-X associated conditions. Elife, 13():(2025) [pubmed] |
Keyword List
submitter keyword: RPS26, human, SILAC |
Contact List
Krzysztof Sobczak |
contact affiliation | Department of Gene Expression Institute of Molecular Biology and Biotechnology Faculty of Biology Adam Mickiewicz University Uniwersytetu Poznanskiego 6 61-614 Poznan |
contact email | ksobczak@amu.edu.pl |
lab head | |
Anna Baud |
contact affiliation | Adam Mickiewicz University in Poznan |
contact email | annbau1@amu.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD047397 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047397
- Label: PRIDE project
- Name: SILAC experiment of HEK 293 cells upon RPS26 silencing