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PXD047386

PXD047386 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRiboproteome remodelling during quiescence exit in Saccharomyces cerevisiae
DescriptionCharacterization of the riboproteome composition in quiescent cells and post-translational reactivation. To characterize ribosome heterogeneity during the exit from quiescence, the protein composition of ribosomal particles from stationary phase and nutrient-stimulated cells was assessed. A label-free quantitative mass spectrometry strategy was taken to compare quiescent yeast cells with cells that had been nutrient stimulated for 30 and 60 min. To this end, crude extracts from these cells were subjected to sucrose gradient centrifugation and three fractions free (F); monosome (M=80S + 60S + 40S) and polysome (P) were analyzed by nano-HPLC-MS/MS. A total of 528 proteins were identified.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:18:06.754.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmittermaria pia valacco
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-29 15:06:20ID requested
12023-12-20 03:37:49announced
22024-10-22 06:18:07announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.ISCI.2023.108727;
Keyword List
submitter keyword: Mass Spectrometry, ribosome, Saccharomyces cerevisiae
Contact List
Paula Portela
contact affiliationUniversidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento Química Biológica, Buenos Aires, Argentina; CONICET Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
contact emailpportela@qb.fcen.uba.ar
lab head
maria pia valacco
contact affiliationUniversity of Buenos Aires CONICET
contact emailpvalacco@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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