PXD047386

PXD047386 is an original dataset announced via ProteomeXchange.

Title	Riboproteome remodelling during quiescence exit in Saccharomyces cerevisiae
Description	Characterization of the riboproteome composition in quiescent cells and post-translational reactivation. To characterize ribosome heterogeneity during the exit from quiescence, the protein composition of ribosomal particles from stationary phase and nutrient-stimulated cells was assessed. A label-free quantitative mass spectrometry strategy was taken to compare quiescent yeast cells with cells that had been nutrient stimulated for 30 and 60 min. To this end, crude extracts from these cells were subjected to sucrose gradient centrifugation and three fractions free (F); monosome (M=80S + 60S + 40S) and polysome (P) were analyzed by nano-HPLC-MS/MS. A total of 528 proteins were identified.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:06.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	maria pia valacco
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-29 15:06:20	ID requested
1	2023-12-20 03:37:49	announced
⏵ 2	2024-10-22 06:18:07	announced	2024-10-22: Updated project metadata.

10.1016/J.ISCI.2023.108727;

submitter keyword: Mass Spectrometry, ribosome, Saccharomyces cerevisiae

Paula Portela
contact affiliation	Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento Química Biológica, Buenos Aires, Argentina; CONICET Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
contact email	pportela@qb.fcen.uba.ar
lab head
maria pia valacco
contact affiliation	University of Buenos Aires CONICET
contact email	pvalacco@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047386

PRIDE project URI

• PRIDE
  1. PXD047386
     1. Label: PRIDE project
     2. Name: Riboproteome remodelling during quiescence exit in Saccharomyces cerevisiae