PXD047386 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Riboproteome remodelling during quiescence exit in Saccharomyces cerevisiae |
Description | Characterization of the riboproteome composition in quiescent cells and post-translational reactivation. To characterize ribosome heterogeneity during the exit from quiescence, the protein composition of ribosomal particles from stationary phase and nutrient-stimulated cells was assessed. A label-free quantitative mass spectrometry strategy was taken to compare quiescent yeast cells with cells that had been nutrient stimulated for 30 and 60 min. To this end, crude extracts from these cells were subjected to sucrose gradient centrifugation and three fractions free (F); monosome (M=80S + 60S + 40S) and polysome (P) were analyzed by nano-HPLC-MS/MS. A total of 528 proteins were identified. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:18:06.754.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | maria pia valacco |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-29 15:06:20 | ID requested | |
1 | 2023-12-20 03:37:49 | announced | |
⏵ 2 | 2024-10-22 06:18:07 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Mass Spectrometry, ribosome, Saccharomyces cerevisiae |
Contact List
Paula Portela |
contact affiliation | Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento Química Biológica, Buenos Aires, Argentina; CONICET Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina |
contact email | pportela@qb.fcen.uba.ar |
lab head | |
maria pia valacco |
contact affiliation | University of Buenos Aires CONICET |
contact email | pvalacco@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047386
- Label: PRIDE project
- Name: Riboproteome remodelling during quiescence exit in Saccharomyces cerevisiae