PXD047378

PXD047378 is an original dataset announced via ProteomeXchange.

Title	Human LD proteome identified by APEX2-mediated proximity labeling
Description	Adipocyte lipid droplets (LDs) play a crucial role in systemic lipid metabolism by storing and releasing lipids to meet the organism's energy needs. Hormonal signals such as catecholamines and insulin act on adipocyte LDs, and impaired responsiveness to these signals can lead to uncontrolled lipolysis, lipotoxicity, and metabolic disease. To investigate the mechanisms that control LD function in human adipocytes, we applied proximity labeling mediated by enhanced ascorbate peroxidase (APEX2) to identify the interactome of PLIN1 in adipocytes differentiated from human mesenchymal progenitor cells. We identified 70 proteins that interact specifically with PLIN1, including PNPLA2 and LIPE, which are the primary effectors of regulated triglyceride hydrolysis, and four members of the 14-3-3 protein family (YWHAB, YWHAE, YWHAZ, and YWHAG), which are known to regulate diverse signaling pathways. Functional studies showed that YWHAB is required for maximum cAMP-stimulated lipolysis, as its CRISPR-Cas9-mediated knockout mitigates lipolysis through a mechanism independent of insulin signaling. These findings reveal a new regulatory mechanism operating in human adipocytes that can impact lipolysis and potentially systemic metabolism.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:40:31.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qin Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-29 06:15:28	ID requested
⏵ 1	2024-01-26 06:40:32	announced

Yang Q, Loureiro ZY, Desai A, DeSouza T, Li K, Wang H, Nicoloro SM, Solivan-Rivera J, Corvera S, Regulation of lipolysis by 14-3-3 proteins on human adipocyte lipid droplets. PNAS Nexus, 2(12):pgad420(2023) [pubmed]

10.1093/pnasnexus/pgad420;

submitter keyword: Human adipocyte, Proximity labeling, Lipid droplet, LC-MS/MS

Silvia Corvera
contact affiliation	Program in Molecular Medicine, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA
contact email	Silvia.Corvera@umassmed.edu
lab head
Qin Yang
contact affiliation	University of Masschusetts Chan Medical School
contact email	qin.yang2@umassmed.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD047378

PRIDE project URI

• PRIDE
  1. PXD047378
     1. Label: PRIDE project
     2. Name: Human LD proteome identified by APEX2-mediated proximity labeling