PXD047341

PXD047341 is an original dataset announced via ProteomeXchange.

Title	Top-down mass spectrometry of native proteoforms and their complexes: A community study
Description	The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-factors. While this approach is powerful, both native mass spectrometry and top-down mass spectrometry are not yet well standardised, and only a limited number of laboratories regularly carry out this type of research. To address this challenge, the Consortium for Top-Down Proteomics (CTDP) initiated a study to develop and test protocols for native mass spectrometry combined with top-down fragmentation of proteins and protein complexes across eleven instruments in nine laboratories. The outcomes are summarised in this report to provide robust benchmarks and a valuable entry point for the scientific community.
HostingRepository	PRIDE
AnnounceDate	2024-05-13
AnnouncementXML	Submission_2024-05-12_23:53:54.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Habeck
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Halobacterium salinarum; NCBI TaxID: 2242; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	6545 Q-TOF LC/MS; Q Exactive; LTQ Orbitrap; Synapt MS; solariX

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-28 08:38:28	ID requested
⏵ 1	2024-05-12 23:53:55	announced

10.1038/S41592-024-02279-6;

submitter keyword: Consortium for Top-Down Proteomics,Native Top-down MS

Frederik Lermyte
contact affiliation	Department of Chemistry, Clemens-Schöpf Institute of Organic Chemistry and Biochemistry, Technical University of Darmstadt
contact email	frederik.lermyte@tu-darmstadt.de
lab head
Tanja Habeck
contact affiliation	Department of Chemistry, Institute of Chemistry and Biochemistry, Technical University of Darmstadt, 64287 Darmstadt
contact email	tanja.habeck@tu-darmstadt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047341

PRIDE project URI

• PRIDE
  1. PXD047341
     1. Label: PRIDE project
     2. Name: Top-down mass spectrometry of native proteoforms and their complexes: A community study