PXD047332

PXD047332 is an original dataset announced via ProteomeXchange.

Title	RNF212B E3 ligase is essential for crossover designation and maturation during male and female meiosis in the mouse
Description	Meiosis, a reductional cell division, relies on precise initiation, maturation and resolution of crossovers (COs) during prophase I to ensure the accurate segregation of homologous chromosomes during metaphase I. This process is regulated by the interplay of RING-E3 ligases such as RNF212 and HEI10 in mammals. In this study, we characterized a novel RNF212B E3 ligase. RNF212B colocalizes and interacts with RNF212 forming small foci along meiotic chromosome cores from zygonema onwards. These consolidate into larger foci at maturing COs, colocalizing with HEI10, CNTD1, and MLH1 at pachynema in a synapsis-dependent and DSB-independent manner. Genetically, RNF212B focus formation depends on the presence of Rnf212 and Msh4 but not on Hei10 and Cntd1, while the unloading of RNF212B at the end of pachynema is dependent on Hei10 and Cntd1. Rnf212b-deficient and enzymatically-dead mutant mice exhibit modest synapsis defects, a reduction in localization of pro-CO factors (MSH4, TEX11, RPA, MZIP2) and absence of MLH1 foci, indicative of nascent COs, resulting in metaphase I cells with mostly univalent chromosomes. Double mutants for Rnf212b and Rnf212 exhibit an identical phenotype to that of Rnf212b single mutant males, while double heterozygous demonstrate a dosage-dependent reduction in the number of COs relative to the single mutant, indicating a functional interplay between both paralogs. SUMOylome analysis of testis from Rnf212b mutants and pull-down analysis of Sumo- and Ubiquitin-tagged HeLa cells, suggest that RNF212B is a novel E3 ligase with Ubiquitin activity, serving as a crucial factor for CO designation and maturation. This conserved pathway holds physiological significance for understanding the biology and homesotais of CO and its role in shaping meiotic recombination landscape.
HostingRepository	PRIDE
AnnounceDate	2024-05-31
AnnouncementXML	Submission_2024-05-31_02:27:39.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Román González-Prieto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-28 07:01:10	ID requested
⏵ 1	2024-05-31 02:27:39	announced

10.1073/PNAS.2320995121;

submitter keyword: Meiosis, SUMO, Ubiquitin

Roman Gonzalez Prieto
contact affiliation	Andalusian Center for Moelcular Biology and Regenerative Medicine (CABIMER), University of Sevilla, Spain
contact email	roman.gonzalez@cabimer.es
lab head
Román González-Prieto
contact affiliation	Andalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville
contact email	roman.gonzalez@cabimer.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047332
     1. Label: PRIDE project
     2. Name: RNF212B E3 ligase is essential for crossover designation and maturation during male and female meiosis in the mouse