PXD047311

PXD047311 is an original dataset announced via ProteomeXchange.

Title	Proteogenomic analysis uncovers neoantigens and bacterial peptides as immunotherapy targets in colorectal cancer
Description	Considerable progress has recently been made in cancer immunotherapy, including immune checkpoint blockade, cancer vaccine, and adoptive T cell methods. The lack of effective targets is a major cause of the low immunotherapy response rate in colorectal cancer (CRC). Here, we used a proteogenomic strategy comprising immunopeptidomics, whole exome sequencing, and 16S ribosomal DNA sequencing analyses of 8 patients with CRC to identify neoantigens and bacterial immunopeptides that can serve as antitumor targets. This study directly identified several personalized neoantigens and bacterial immunopeptides. Immunoassays showed that all neoantigens and 5 of 8 bacterial immunopeptides could be recognized by autologous T cells. Additionally, T cell receptor (TCR) αβ sequencing revealed the TCR repertoire of epitope-reactive CD8+ T cells. Functional studies showed that T cell receptor -T (TCR-T) could be activated by epitope pulsed lymphoblastoid cells. Overall, this study comprehensively profiled the CRC immunopeptidome, revealing several neoantigens and bacterial immunopeptides with potential to serve as immunotherapy targets in CRC.
HostingRepository	iProX
AnnounceDate	2023-11-28
AnnouncementXML	Submission_2023-11-28_00:03:17.799.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pengju Yao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-28 00:02:51	ID requested
⏵ 1	2023-11-28 00:03:18	announced

Dataset with its publication pending

submitter keyword: CRC, immunopeptidome, HLA, epitope, neoantigen, bacterial peptides

Jianguo Ji
contact affiliation	Peking University
contact email	jijg@pku.edu.cn
lab head
Pengju Yao
contact affiliation	Peking University
contact email	1801110534@pku.edu.cn
dataset submitter

iProX dataset URI