PXD047309

PXD047309 is an original dataset announced via ProteomeXchange.

Title	N-glycoproteomics of CHO cell-expressed FGFR3
Description	Receptor tyrosine kinases (RTKs) that undergo N-linked glycosylation undergo protein quality control in the endoplasmic reticulum (ER) and subsequently transfer to the Golgi apparatus, where they acquire a diverse array of glycan structures. The family of receptor tyrosine kinases (RTKs) known as FGFR (Fibroblast Growth Factor Receptor) possesses N-glycosylation potential sites within their extracellular domains. Mutations in the modification site of FGFR3 have been documented in conditions such as Achondroplasia (ACH) and hypochondroplasia (HCH). While it is believed that ACH and HCH are associated with the overactivity of FGFR3 and accumulation in the ER, there has yet to be a case where the root cause has been explained from the perspective of glycosylation. This study furnishes a site-specific profile of N-glycans on FGFR3 through structural analysis of the extracellular domain of FGFR3 containing N-glycosylation potential sites, conducted via nLC-MS/MS. Moreover, we generated mutants of FGFR3 with a single glycan deficiency at each site and examined the impact of N-glycans at these sites on the receptor. The findings demonstrated reduced phosphorylation in the N98 glycosylation deficient mutant and accumulation in the ER in the N262 glycosylation deficient mutant, indicating ligand-independent autophosphorylation of the receptor. Hence, it is proposed that N-glycans in FGFR3 may play a role in functional regulation, offering fresh perspectives on disease etiology from a glycosylation standpoint.
HostingRepository	PRIDE
AnnounceDate	2026-08-05
AnnouncementXML	Submission_2026-08-04_17:43:04.060.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD047309
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Naoki Fujitani
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 22:32:54	ID requested
⏵ 1	2026-08-04 17:43:04	announced

10.6019/PXD047309;

10.1016/j.bbagen.2024.130565;

Hashimoto U, Fujitani N, Uehara Y, Okamoto H, Saitou A, Ito F, Ariki S, Shiratsuchi A, Hasegawa Y, Takahashi M, N-glycan on N262 of FGFR3 regulates the intracellular localization and phosphorylation of the receptor. Biochim Biophys Acta Gen Subj, 1868(4):130565(2024) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Glycoproteomics (B/D-HPP)

submitter keyword: FGFR3, fibroblast growth factor receptor 3,N-glycoproteomics

Motoko Takahashi
contact affiliation	Sapporo Medical University School of Medicine
contact email	takam@sapmed.ac.jp
lab head
Naoki Fujitani
contact affiliation	Sapporo Medical University
contact email	fujitani@sapmed.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047309
     1. Label: PRIDE project
     2. Name: N-glycoproteomics of CHO cell-expressed FGFR3