PXD047298

PXD047298 is an original dataset announced via ProteomeXchange.

Title	The 26S proteasome substrates and the dynamics of nuclear proteome upon DNA damage
Description	Upon DNA damage, numerous proteins are targeted for ubiquitin-dependent proteasomal degradation. We show that phosphorylation of Rpn10/PSMD4 (ubiquitin receptor of the proteasome) at Ser266, is required for DNA repair. Here, we performed TurboID proximity labeling for capturing the proteasome substrates under DNA damage. Numerous proteins which involve in DNA damage response, chromosome reorganization cell cycle and histone modification were identified as the potential pronuteasome substrates. We find that DNA damage reduced the overall affinity between substrates and wild-type Rpn10 compared to Rpn10-Ser266 mutant cells. Combining TMT quantitative proteomic studies, we observed the dynamics of more than 2,000 nuclear proteins upon DNA damage and time course release. Most proteins were accumulated in wild-type cells in the time course release, while the Rpn10-Ser266 mutant cells show almost no changes in protein level. These findings reveal an inherent self-limiting mechanism of the proteasome that, by controlling substrate recognition through Rpn10 phosphorylation, fine-tunes protein degradation for optimal responses under stress.
HostingRepository	iProX
AnnounceDate	2023-11-28
AnnouncementXML	Submission_2023-11-27_19:31:51.581.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yina Tian
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 19:30:48	ID requested
⏵ 1	2023-11-27 19:31:51	announced

Dataset with its publication pending

submitter keyword: DNA damage, nuclear proteome, proteasome substrate, TurboID, TMT

Xing Guo
contact affiliation	Zhejiang University
contact email	xguo@zju.edu.cn
lab head
Yina Tian
contact affiliation	Zhejiang University
contact email	tyzhu@zju.edu.cn
dataset submitter

iProX dataset URI