PXD047298
PXD047298 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The 26S proteasome substrates and the dynamics of nuclear proteome upon DNA damage |
Description | Upon DNA damage, numerous proteins are targeted for ubiquitin-dependent proteasomal degradation. We show that phosphorylation of Rpn10/PSMD4 (ubiquitin receptor of the proteasome) at Ser266, is required for DNA repair. Here, we performed TurboID proximity labeling for capturing the proteasome substrates under DNA damage. Numerous proteins which involve in DNA damage response, chromosome reorganization cell cycle and histone modification were identified as the potential pronuteasome substrates. We find that DNA damage reduced the overall affinity between substrates and wild-type Rpn10 compared to Rpn10-Ser266 mutant cells. Combining TMT quantitative proteomic studies, we observed the dynamics of more than 2,000 nuclear proteins upon DNA damage and time course release. Most proteins were accumulated in wild-type cells in the time course release, while the Rpn10-Ser266 mutant cells show almost no changes in protein level. These findings reveal an inherent self-limiting mechanism of the proteasome that, by controlling substrate recognition through Rpn10 phosphorylation, fine-tunes protein degradation for optimal responses under stress. |
HostingRepository | iProX |
AnnounceDate | 2023-11-28 |
AnnouncementXML | Submission_2023-11-27_19:31:51.581.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yina Tian |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-27 19:30:48 | ID requested | |
⏵ 1 | 2023-11-27 19:31:51 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: DNA damage, nuclear proteome, proteasome substrate, TurboID, TMT |
Contact List
Xing Guo | |
---|---|
contact affiliation | Zhejiang University |
contact email | xguo@zju.edu.cn |
lab head | |
Yina Tian | |
contact affiliation | Zhejiang University |
contact email | tyzhu@zju.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |