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PXD047298

PXD047298 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe 26S proteasome substrates and the dynamics of nuclear proteome upon DNA damage
DescriptionUpon DNA damage, numerous proteins are targeted for ubiquitin-dependent proteasomal degradation. We show that phosphorylation of Rpn10/PSMD4 (ubiquitin receptor of the proteasome) at Ser266, is required for DNA repair. Here, we performed TurboID proximity labeling for capturing the proteasome substrates under DNA damage. Numerous proteins which involve in DNA damage response, chromosome reorganization cell cycle and histone modification were identified as the potential pronuteasome substrates. We find that DNA damage reduced the overall affinity between substrates and wild-type Rpn10 compared to Rpn10-Ser266 mutant cells. Combining TMT quantitative proteomic studies, we observed the dynamics of more than 2,000 nuclear proteins upon DNA damage and time course release. Most proteins were accumulated in wild-type cells in the time course release, while the Rpn10-Ser266 mutant cells show almost no changes in protein level. These findings reveal an inherent self-limiting mechanism of the proteasome that, by controlling substrate recognition through Rpn10 phosphorylation, fine-tunes protein degradation for optimal responses under stress.
HostingRepositoryiProX
AnnounceDate2023-11-28
AnnouncementXMLSubmission_2023-11-27_19:31:51.581.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYina Tian
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-27 19:30:48ID requested
12023-11-27 19:31:51announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DNA damage, nuclear proteome, proteasome substrate, TurboID, TMT
Contact List
Xing Guo
contact affiliationZhejiang University
contact emailxguo@zju.edu.cn
lab head
Yina Tian
contact affiliationZhejiang University
contact emailtyzhu@zju.edu.cn
dataset submitter
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