PXD047294

PXD047294 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Proteomics Analysis of Bovine Sperm Head Plasma Membrane Associated with Fertility
Description	Bull fertility impacts herd fertility, but accurately predicting male fertility from sperm characteristics is difficult once extremes are removed. The objectives of this study are identification, relative quantification, and comparison of sperm head plasma membrane (HPM) proteomics in bulls of differing bull fertility index (BFI). HPM from one fresh ejaculate from 16 Holstein bulls (8 each high and low fertility; HF, LF) was extracted and its proteins were assessed by tandem mass spectrometry. Mass spectra were aligned to UniProtKB mammal database and proteins were identified and characterized by SpectrumMill. Of 22,117 total proteins identified (unique plus homologous; 1% false discovery rate), Mass Profiler Professional statistical analysis identified 67 differentially abundant proteins (DAPs) in the HPM of 3 bulls each with highest and lowest BFI (105.66±0.54, 91.33±1.44, respectively; p<0.01]. DAPs differed in intensity by at least 2-fold (48 up-regulated, 19 down-regulated, HF vs LF) and meta-analysis confirmed their association to BFI. Gene ontology assigned up-regulated proteins to sperm fertilization mechanisms, and down-regulated proteins to catalytic and transporter activity. Linear regression positively correlated BFI of the 6 HF and LF bulls to all up-regulated DAPs (r2=0.65 to 0.97, p≤0.05), and negatively correlated BFI to all down-regulated DAPs (r2=0.76 to 0.96, p<0.05). In the 16-bull population, linear regression positively correlated BFI to 38 of the up-regulated DAPs (r2=0.29 to 0.66; p≤0.05), and negatively correlated 6 of the down-regulated DAPs (r2=0.26 to 0.44; p≤0.05). In summary, this study revealed proteins in HPM, highlighting their significant role in sperm fertilization and correlation with bull fertility.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_17:42:52.238.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulos Chumala
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 16:49:49	ID requested
⏵ 1	2026-06-07 17:42:52	announced

10.1038/s41598-025-34626-8;

Imran M, Buhr MM, Chumala P, Katselis GS, Comprehensive proteomics analysis of bovine sperm head plasma membrane associated with fertility. Sci Rep, 16(1):(2026) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Fertility biomarkers, Proteomics analysis,Bull fertility, Sperm head plasma membrane

Dr. George Katselis
contact affiliation	Assistant Professor, Mass spectrometry Facility, Canadian Centre for Health and Safety in Agriculture, College of Medine, University of Saskatchewan.
contact email	george.katselis@usask.ca
lab head
Paulos Chumala
contact affiliation	Canadian Centre for Rural and Agricultural Heatlh , College of Medicine, University of Saskatchewan
contact email	pab776@mail.usask.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD047294

PRIDE project URI

• PRIDE
  1. PXD047294
     1. Label: PRIDE project
     2. Name: Comprehensive Proteomics Analysis of Bovine Sperm Head Plasma Membrane Associated with Fertility