PXD047280

PXD047280 is an original dataset announced via ProteomeXchange.

Title	Bisphenol A negatively impacts human sperm microRNA and protein profiles
Description	Bisphenol A (BPA), a widely prevalent endocrine disruptor, has detrimental effects on human health and male reproduction. Elevated BPA levels have been linked to reduced sperm quality and production, while paternal exposure may result in poor reproductive outcomes and offspring health. Nevertheless, the impact of BPA on human sperm miRNA and protein profiles and its implications for fertilization and post-fertilization events remain unexplored. This study investigates the association between BPA concentration in seminal plasma and seminal quality and examines alterations in human sperm miRNA and protein expression among men with varying BPA levels in seminal plasma. BPA levels were measured in seminal plasma from 102 Portuguese men, and it was detected in 88% of the samples. No correlation was observed between BPA levels and age or seminal parameters. The small RNA content of 15 normozoospermic samples was evaluated using small RNA sequencing, revealing that 15 miRNAs exhibited a correlation with BPA levels. Gene ontology analysis of their target genes indicated their involvement in embryonic development and response to stress. The proteome of 20 normozoospermic human sperm samples, categorized into four groups based on BPA levels, was accessed by liquid chromatography-mass spectrometry. Sixty-two differentially expressed proteins were identified across groups. The biological processes most affected in samples with higher BPA levels were "protein sumoylation" and "cytoplasmic translation”. The altered miRNAs and proteins identified in spermatozoa from men environmentally exposed to BPA represent potential biological markers of exposure to this endocrine disruptor and may help elucidate cases of idiopathic male infertility, fertilization failure, and abnormal embryo development.
HostingRepository	PRIDE
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-16_23:43:39.491.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD047280
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sara Dufour
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 07:50:36	ID requested
⏵ 1	2025-06-16 23:43:39	announced

10.6019/PXD047280;

10.1007/S12403-024-00627-7;

submitter keyword: Bisphenol A

seminal plasma

spermatozoa

male infertility

proteome

microRNA

Joana Santiago
contact affiliation	Department of Medical Sciences, Institute of Biomedicine, University of Aveiro, 3810-193, Aveiro, Portugal
contact email	joanasantiago@ua.pt
lab head
Sara Dufour
contact affiliation	VIB-UGent
contact email	sara.dufour@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047280
     1. Label: PRIDE project
     2. Name: Bisphenol A negatively impacts human sperm microRNA and protein profiles