PXD047268

PXD047268 is an original dataset announced via ProteomeXchange.

Title	Probing the trypanosome nuclear pore by TurboID proximity labelling
Description	Previous BioID experiments targeting the nucleoporin (NUP) NUP158 and the nuclear transport factor MEX67 indicated (PMID: 36410438; PXD031245) that proximity labelling delivers highly specific interactome data in the confined localisation of the nuclear pore, with a labelling radius well below the size of the nuclear pore. Here we extended this approach to target NUPs NUP110, NUP76 and NUP96 with a TurboID-HA tandem tag fused to both, the N and C-terminus of each respective target protein. While the central NUP96 biotinylated most nuclear pore proteins with the marked exception of the nuclear basket, NUP158, NUP110 and NUP76 caused biotinylation of specific sub-complexes of the pore and Mex67 labelled NUPs lining the pore channel.
HostingRepository	PRIDE
AnnounceDate	2023-12-04
AnnouncementXML	Submission_2023-12-04_03:58:06.521.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Zoltner
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 04:01:47	ID requested
⏵ 1	2023-12-04 03:58:07	announced

Moreira CMDN, Kelemen CD, Obado SO, Zahedifard F, Zhang N, Holetz FB, Gauglitz L, Dallagiovanna B, Field MC, Kramer S, Zoltner M, Impact of inherent biases built into proteomic techniques: Proximity labeling and affinity capture compared. J Biol Chem, 299(1):102726(2023) [pubmed]

10.1016/j.jbc.2022.102726;

submitter keyword: BioID, Trypanosoma brucei, porin, nuclear transport, nuclear pore

Dr. Martin Zoltner
contact affiliation	Head of Drug Discovery and Evaluation Charles University in Prague, BIOCEV Průmyslová 595 252 50 Vestec Czech Republic phone: +420 325 873 966
contact email	zoltnerm@natur.cuni.cz
lab head
Martin Zoltner
contact affiliation	Division of Biological Chemistry & Drug Discovery School of Life Sciences University of Dundee Dundee DD1 5EH
contact email	m.zoltner@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD047268

PRIDE project URI

• PRIDE
  1. PXD047268
     1. Label: PRIDE project
     2. Name: Probing the trypanosome nuclear pore by TurboID proximity labelling