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PXD047262

PXD047262 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCombined transcriptome and proteome profiling indicate cell-type-specific functions of Drosophila garland and pericardial nephrocytes
DescriptionDrosophila nephrocytes are specialised cells that share critical functional, morphological, and molecular features with podocytes of the mammalian kidney, including the slit diaphragm, which is a functional filtration barrier. Accordingly, nephrocytes are considered highly suitable invertebrate models for glomerular disease in humans. In this study, we established a method to individually isolate the garland and pericardial nephrocytes from Drosophila of different developmental stages. This allowed us to collect nephrocyte samples that were uncontaminated by other cell types. We used a combination of mass spectrometry-based proteomics and RNA-Seq-based transcriptomics to characterise the proteome and transcriptome of the respective cells in an integrated manner. In addition to identifying a set of factors that were expressed exclusively in nephrocytes, we observed characteristic changes in the cellular proteome and transcriptome as a consequence of ageing. Furthermore, functional enrichment analyses suggested that larval and adult nephrocytes fulfil distinct physiological functions, which was also indicated for garland and pericardial nephrocytes in adult animals. In addition, the latter cells were characterised by transcriptomic and proteomic profiles that were suggestive of a cell-type-specific energy metabolism. Finally, both types of nephrocytes displayed transcriptomic signatures that indicated elevated immune signalling. The data generated in this study may represent a valuable basis for a more specific application of the Drosophila model system in the analysis of renal cell function in health and disease.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_07:00:52.433.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHeiko Harten
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-27 02:54:43ID requested
12024-10-08 05:52:31announced
22024-10-22 07:00:52announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: labyrinth channels, transcriptome,Ageing, kidney model, nephropathies, nephrocytes, slit diaphragms, proteome, Drosophila melanogaster
Contact List
Heiko Harten
contact affiliationUniversity of Osnabrück, Department of Zoology & Developmental Biology, 49076 Osnabrück, Germany
contact emailHarten@biologie.uni-osnabrueck.de
lab head
Heiko Harten
contact affiliationOsnabruck University
contact emailHarten@biologie.uni-osnabrueck.de
dataset submitter
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Dataset FTP location
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