PXD047262

PXD047262 is an original dataset announced via ProteomeXchange.

Title	Combined transcriptome and proteome profiling indicate cell-type-specific functions of Drosophila garland and pericardial nephrocytes
Description	Drosophila nephrocytes are specialised cells that share critical functional, morphological, and molecular features with podocytes of the mammalian kidney, including the slit diaphragm, which is a functional filtration barrier. Accordingly, nephrocytes are considered highly suitable invertebrate models for glomerular disease in humans. In this study, we established a method to individually isolate the garland and pericardial nephrocytes from Drosophila of different developmental stages. This allowed us to collect nephrocyte samples that were uncontaminated by other cell types. We used a combination of mass spectrometry-based proteomics and RNA-Seq-based transcriptomics to characterise the proteome and transcriptome of the respective cells in an integrated manner. In addition to identifying a set of factors that were expressed exclusively in nephrocytes, we observed characteristic changes in the cellular proteome and transcriptome as a consequence of ageing. Furthermore, functional enrichment analyses suggested that larval and adult nephrocytes fulfil distinct physiological functions, which was also indicated for garland and pericardial nephrocytes in adult animals. In addition, the latter cells were characterised by transcriptomic and proteomic profiles that were suggestive of a cell-type-specific energy metabolism. Finally, both types of nephrocytes displayed transcriptomic signatures that indicated elevated immune signalling. The data generated in this study may represent a valuable basis for a more specific application of the Drosophila model system in the analysis of renal cell function in health and disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:52.433.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Heiko Harten
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	amidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-27 02:54:43	ID requested
1	2024-10-08 05:52:31	announced
⏵ 2	2024-10-22 07:00:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: labyrinth channels, transcriptome,Ageing, kidney model, nephropathies, nephrocytes, slit diaphragms, proteome, Drosophila melanogaster

Heiko Harten
contact affiliation	University of Osnabrück, Department of Zoology & Developmental Biology, 49076 Osnabrück, Germany
contact email	Harten@biologie.uni-osnabrueck.de
lab head
Heiko Harten
contact affiliation	Osnabruck University
contact email	Harten@biologie.uni-osnabrueck.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD047262
     1. Label: PRIDE project
     2. Name: Combined transcriptome and proteome profiling indicate cell-type-specific functions of Drosophila garland and pericardial nephrocytes