Update information. We designed novel calibrators that include three phosphorylated Tau sites: Tau (1-22)-pT231, Tau (1-22)-pT217, and Tau (1-22)-pT181, respectively. Our synthesized peptides have various advantages compared with GSK3β-phosphorylated Tau-441. The novel fully synthesized calibrators not only improved the precision and stability of immunoassays but also served as potential calibrators for the diagnosis of AD.